Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca
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Data
1998-06-01
Autores
Morais, Glaucia Almeida de
Takaki, Massanori [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Brazilian Archives of Biology and Technology
Resumo
Determination of α- and β-amylase activity in the extracts of cotyledons of Phaseolus vulgaris L. cv. cariocawas done using selective inactivation of α-amylase by lowering the pH of the incubation medium or by the use of EDTA as inhibitor or selective inactivation of β-amylase by the use of HgCl2 or by heating to 70ºC in the presence of CaCl2; and still by using the reagent starch azure for specific determination of α-amylase. Results indicated that the methods used were inappropriate in this case, being indicated the determination of total amylase activity.
Descrição
Palavras-chave
determination, selective inactivation, Phaseolus vulgaris
Como citar
Brazilian Archives of Biology and Technology. Brazilian Archives of Biology and Technology, v. 41, n. 1, p. 17-25, 1998.