New crystal forms of Diocleinae lectins in the presence of different dimannosides
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2006-11-01
Autores
Mendes Batista Moreno, Frederico Bruno
Bezerra, Gustavo Arruda
Maia de Oliveira, Taiana
Prata de Souza, Emmanuel
Matias da Rocha, Bruno Anderson
Benevides, Raquel Guimaraes
Delatorre, Plinio
Cavada, Benildo Sousa
Filgueira de Azevedo, Walter
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Blackwell Publishing
Resumo
Studying the interactions between lectins and sugars is important in order to explain the differences observed in the biological activities presented by the highly similar proteins of the Diocleinae subtribe. Here, the crystallization and preliminary X--ray data of Canavalia gladiata lectin (CGL) and C. maritima lectin (CML) complexed with Man(alpha 1-2)Man(alpha 1)OMe, Man(alpha 1-3)Man(alpha 1)OMe and Man(alpha 1-4)Man(alpha 1)OMe in two crystal forms [the complexes with Man(alpha 1-3)Man(alpha 1)OMe and Man(alpha 1-4)Man(alpha 1)OMe crystallized in space group P3(2) and those with Man(alpha 1-2)Man(alpha 1)OMe crystallized in space group I222], which differed from those of the native proteins (P2(1)2(1)2 for CML and C222 for CGL), are reported. The crystal complexes of ConA-like lectins with Man(alpha 1-4)Man(alpha 1)OMe are reported here for the first time.
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Acta Crystallographica Section F-structural Biology and Crystallization Communications. Oxford: Blackwell Publishing, v. 62, p. 1100-1103, 2006.