Immunodiagnosis of swine cysticercosis by indirect ELISA employing a heterologous antigen from Taenia crassiceps metacestode
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Data
1996-09-16
Autores
Biondi, Germano Francisco [UNESP]
Mucciolo, R. G.
Nunes, Caris Maroni [UNESP]
Richtzenhain, L. J.
Título da Revista
ISSN da Revista
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Editor
Elsevier B.V.
Resumo
C. Larralde et al. (1990, Arch. Pathol. Lab. Med., 114:926-928) demonstrated that heterologous antigen from the laboratory-adapted murine Taenia crassiceps metacestode may substitute those from Taenia solium in the immunodiagnosis of human cysticercosis by the indirect enzyme-linked immunosorbent assay (IE). ?This antigen is easily obtained at a laboratory level and solves the problem of T. solium cysticerci collection from naturally or experimentally infected swine. In this study an IE employing a heterologous antigen from the T. crassiceps metacestode was evaluated for the immunodiagnosis of swine cysticercosis. Sera from 300 swine free of T. solium cysticerci by post-mortem examination were employed to determine two IE cut- off values: 1) Mean ELISA values + 2 standard deviations (2 sigma cut-off) and 2) - Mean ELISA values + 3 standard deviations (3 sigma cut-off). The specificity of IE was 97% with the 2 sigma cut-off and 100% with the 3 sigma cut-off. When applied to ten sera from swine infected by cysticerci of T. solium by post-mortem examination, the sensitivity of IE was 100% independent of the cut off.
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Palavras-chave
swine cysticercosis, immunodiagnosis, indirect ELISA, heterologous antigen, Taenia crassiceps
Como citar
Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 64, n. 4, p. 261-266, 1996.