Emprego da linhagem selvagem de Candida albicans isolada de efluente industrial para a avaliação da biosorção/biodegradação do corante azóico direct violet 51
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Data
2005-03-09
Autores
Vitor, Vivian [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
A presente pesquisa enfoca a utilização da levedura Candida albicans, isolada de um efluente industrial, em processos de remoção de corantes azóicos. Neste estudo o corante escolhido foi da classe dos diretos, o Direct Violet 51 em solução aquosa. A levedura selvagem foi utilizada na forma de suspensão no estudo da biosorção do corante em diferentes valores de pH comparando o comportamento das células não autoclavadas e autoclavadas, objetivando a remoção do corante em solução aquosa. O corante Direct Violet 51 foi analisado quanto a sua estabilidade espectral em diferentes valores de pH e melhor pH biosortivo, mostrando-se praticamente estável em termos espectrais e o valor de pH 2,5 foi o que melhor se mostrou ativo quanto a capacidade de remover o corante frente as biomassas não autoclavadas e autoclavada de Candida albicans. Em todos os experimentos de interação biosortiva realizados entre a biomassa e o corante, as células autoclavadas foram as que apresentaram uma maior capacidade biosortiva em relação as células não autoclavadas. A avaliação do potencial adsortivo da levedura foi analisado de acordo com as Equações das Isotermas de Adsorção de Freundlich e Langmuir. A comparação entre os coeficientes de correlação das Isotermas de Adsorção indicam que a equação de Langmuir é a que melhor explica a interação da levedura com o corante Direct Violet 51 em todos os valores de pH. O estudo comparativo realizado entre as leituras das amostras pelo aparelho UV-VIS e pelo FT-IR mostraram que há pequenas alterações na estrutura do corante podendo ser um indício de biodegradação. Pelos dados apresentados, o que de fato podemos afirmar é que, do ponto de vista da biosorção, a levedura Candida albicans demonstra habilidade na remoção de substâncias coloridas, tendo potencial enzimático capaz de alterar as estruturas químicas destes pigmentos coloridos que são encontrados em efluentes industriais.
The present research focuses the utilization of yeast Candida albicans, isolate of a industrial effluent, in processes of removal of azo-dyes. In this study, the dye used was the Direct Violet 51 that belong the classroom of the direct dyes. The wild yeast was used in the suspension form in the study of the biosorption of the dye in different values of pH, comparing the behavior of the autoclaved cells and not autoclaved cells, objectifying the removal of the dye in aqueous solutions. The dye Direct Violet 51 was analyzed in its spectral stability in different values of pH and was found the better biosorptive pH value, revealing practically steady in spectral terms, and the value of pH 2,50 was what it presented greater capacity to remove the dye front the autoclaved cells and not autoclaved cells of the Candida albicans. In all the experiments of biosorptive interaction carried through between the biomass and the dye, the autoclaved cells had been the ones that had presented a greater biosorptive capacity in relation the not autoclaved cells. The evaluation of the adsorptive potential of the yeast was analyzed in accordance with the equations of the isotherms of adsorption of Freundlich and Langmuir. The comparison between the coefficients of correlation of the Isotherms of Adsorption indicates that the equation of Langmuir is the one that better explains the interaction of the yeast with the dye Direct Violet 51 in all values of pH. The carried through comparative study it between the readings of the samples for the device UV-VIS and for FT-IR, they had shown that it has small alterations in the structure of the dye, being able to be a biodegradation indication. For the obtained data, what we can affirm is that, of the point of view of the biosorption, the yeast Candida albicans demonstrates ability in the colorful substance... (Complete abstract, click electronic address below)
The present research focuses the utilization of yeast Candida albicans, isolate of a industrial effluent, in processes of removal of azo-dyes. In this study, the dye used was the Direct Violet 51 that belong the classroom of the direct dyes. The wild yeast was used in the suspension form in the study of the biosorption of the dye in different values of pH, comparing the behavior of the autoclaved cells and not autoclaved cells, objectifying the removal of the dye in aqueous solutions. The dye Direct Violet 51 was analyzed in its spectral stability in different values of pH and was found the better biosorptive pH value, revealing practically steady in spectral terms, and the value of pH 2,50 was what it presented greater capacity to remove the dye front the autoclaved cells and not autoclaved cells of the Candida albicans. In all the experiments of biosorptive interaction carried through between the biomass and the dye, the autoclaved cells had been the ones that had presented a greater biosorptive capacity in relation the not autoclaved cells. The evaluation of the adsorptive potential of the yeast was analyzed in accordance with the equations of the isotherms of adsorption of Freundlich and Langmuir. The comparison between the coefficients of correlation of the Isotherms of Adsorption indicates that the equation of Langmuir is the one that better explains the interaction of the yeast with the dye Direct Violet 51 in all values of pH. The carried through comparative study it between the readings of the samples for the device UV-VIS and for FT-IR, they had shown that it has small alterations in the structure of the dye, being able to be a biodegradation indication. For the obtained data, what we can affirm is that, of the point of view of the biosorption, the yeast Candida albicans demonstrates ability in the colorful substance... (Complete abstract, click electronic address below)
Descrição
Palavras-chave
Biofísica, Despoluição ambiental, Biosorção, Biodegradação, Biorremoção, Biosorption
Como citar
VITOR, Vivian. Emprego da linhagem selvagem de Candida albicans isolada de efluente industrial para a avaliação da biosorção/biodegradação do corante azóico direct violet 51. 2005. xi, 104 f. Dissertação (mestrado) - Universidade Estadual Paulista, Instituto de Biociências, 2005.