Diagrammatic scale for evaluation of Bremia lactucae sporulation in lettuce seedlings

dc.contributor.authorJacinto, Ana Carolina Pires
dc.contributor.authorCastoldi, Renata
dc.contributor.authorBraz, Leila Trevisan [UNESP]
dc.contributor.institutionUniversidade Federal de Uberlândia (UFU)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T16:12:20Z
dc.date.available2023-07-29T16:12:20Z
dc.date.issued2023-01-01
dc.description.abstractIn disease assessment, the use of diagrammatic scales provides a standard reference point for comparison. There is still no precise and accurate scale for the evaluation of sporulation intensity of downy mildew in lettuce seedlings. The objective of this study was to propose a diagrammatic scale for the evaluation of downy mildew severity in lettuce seedlings. Thirty lettuce seedlings with different sporulation intensities were used to develop the proposed diagrammatic scale. Solaris lettuce cultivar was sown, and fifteen days after sowing, inoculations occurred using distilled water, the pathogen sporangia, and Tween 20. The monitoring of disease development was performed daily.When the first sporulation appeared on the cotyledonaryleaves,the proposed scale was verified to meet the minimum and maximum levels of disease severity. Two evaluators verified the accuracy, precision and performed the estimation of the sporulation and reproducibility using a scale. The images obtained are important in the standardization of a scale to identify resistant, tolerant and susceptible plants to downy mildew. Furthermore, it allows validating a diagrammatic scale of sporulation intensity of B. lactucae in lettuce seedlings. Thus, it is the diagrammatic scale developed in the work will guide future research to accelerate the detection of mildew severity in lettuce seedlingsen
dc.description.affiliationUniversidade Federal de Uberlândia Campus Umuarama, Minas Gerais
dc.description.affiliationUniversidade Federal de Uberlândia Campus Monte Carmelo, Minas Gerais
dc.description.affiliationUniversidade Estadual Paulista “Júlio de Mesquita Filho” Departamento de Produção Vegetal, São Paulo
dc.description.affiliationUnespUniversidade Estadual Paulista “Júlio de Mesquita Filho” Departamento de Produção Vegetal, São Paulo
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent132-135
dc.identifierhttp://dx.doi.org/10.1590/0034-737X202370020015
dc.identifier.citationRevista Ceres, v. 70, n. 2, p. 132-135, 2023.
dc.identifier.doi10.1590/0034-737X202370020015
dc.identifier.issn2177-3491
dc.identifier.issn0034-737X
dc.identifier.scopus2-s2.0-85153716671
dc.identifier.urihttp://hdl.handle.net/11449/249900
dc.language.isoeng
dc.relation.ispartofRevista Ceres
dc.sourceScopus
dc.subjectdisease severity
dc.subjectdowny mildew
dc.subjectLactuca sativa
dc.subjectrating scale
dc.titleDiagrammatic scale for evaluation of Bremia lactucae sporulation in lettuce seedlingsen
dc.typeArtigo
unesp.author.orcid0000-0001-9406-0917[2]

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