The materno-fetal interface in llama (Lama guanicoe glama)

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dc.contributor.authorIturrizaga, David M.
dc.contributor.authorVerechia, Flavia T. [UNESP]
dc.contributor.authorSantos, Tatiana C.
dc.contributor.authorBombonato, Pedro P.
dc.contributor.authorTeixeira, Dulcinéa G.
dc.contributor.authorMiglino, Maria A.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionCidade Universitária
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:22:29Z
dc.date.available2014-05-27T11:22:29Z
dc.date.issued2007-06-01
dc.description.abstractSamples from 9 llamas (28 through 36 weeks of gestation) were collected and fixed in 4% buffered paraformaldehyde (light microscopy) and in 2.5% buffered glutaraldehyde (transmission and scanning electron microscopy). The material was processed in paraplast and slides (5μm) were stained with HE, PAS, Masson-Trichrome, acid phosphatase and Perl's. The uteroferrin was immunolocalized. The results show that llama placenta is chorioallantoic, diffuse, folded and epitheliochorial, and the fetus is covered with an epidermal membrane. The trophoblast cells have variable morphology: cubic, rounded and triangular cells, with cytoplasm containing PAS-positive granules. Binucleated cells with large cytoplasm and rounded nuclei, as well as giant trophoblastic cells with multiple nuclei were also observed. Numerous blood vessels were observed beneath the cells of the uterine epithelium and around the chorionic subdivided branches. Glandular activity was shown by PAS, Perl's, and acid phosphatase positive reactions in the cytoplasm and glandular lumen, and by immunolocalization of the uteroferrin in the glandular epithelium. The uterine glands open in spaces formed by the areoles, which are filled by PAS-positive material. The llama fetus was covered by the epidermal membrane, composed of stratified epithelium, with up to seven layers of mono-, bi- or trinucleated cells. The high level of maternal and fetal vascularization surfaces indicates an intense exchange of substances across both surfaces. The metabolic activity shown in the uterine glands suggests an adaptation of the gestation to the high altitudes of the natural habitat of this species.en
dc.description.affiliationDepartamento de Cirurgia Faculdade de Medicina Veterinária e Zootecnia Universidade de São Paulo, Av. Prof. Dr. O. Marques de Paiva 87, São Paulo, SP 05508-270
dc.description.affiliationDepto. Cirurgia Faculdade de Medicina Veterinária e Zootecnia Cidade Universitária, Av. Prof. Dr. O. Marques de Paiva 87, São Paulo, SP 05508-270
dc.description.affiliationUniversidade Estadual Paulista Julio de Mesquita Filho Unesp Campus de Dracena, Rodov. Cmte Joao Ribeiro de Barros, Dracena, SP 17900-000
dc.description.affiliationUnespUniversidade Estadual Paulista Julio de Mesquita Filho Unesp Campus de Dracena, Rodov. Cmte Joao Ribeiro de Barros, Dracena, SP 17900-000
dc.format.extent221-228
dc.identifierhttp://dx.doi.org/10.1590/S0100-736X2007000600001
dc.identifier.citationPesquisa Veterinaria Brasileira, v. 27, n. 6, p. 221-228, 2007.
dc.identifier.doi10.1590/S0100-736X2007000600001
dc.identifier.file2-s2.0-34548681214.pdf
dc.identifier.issn0100-736X
dc.identifier.issn1678-5150
dc.identifier.scieloS0100-736X2007000600001
dc.identifier.scopus2-s2.0-34548681214
dc.identifier.urihttp://hdl.handle.net/11449/69689
dc.identifier.wosWOS:000249011000001
dc.language.isoeng
dc.relation.ispartofPesquisa Veterinária Brasileira
dc.relation.ispartofjcr0.385
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectEphiteliochorial
dc.subjectLlama
dc.subjectMorphology
dc.subjectPlacenta
dc.subjectTrophoblast
dc.subjectUteroferrin
dc.subjectLama (mammal)
dc.subjectLama glama
dc.titleThe materno-fetal interface in llama (Lama guanicoe glama)en
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/pvb/paboutj.htm
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Tecnológicas, Dracenapt
unesp.departmentZootecnia - FCATpt

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