Cytogenetic analysis in the spermatogenesis of Triatoma melanosoma (Reduviidae; Heteroptera)

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dc.contributor.authorBardella, V. B. [UNESP]
dc.contributor.authorOliveira, Maria Tercília Vilela de Azeredo [UNESP]
dc.contributor.authorTartarotti, E. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:23:37Z
dc.date.available2014-05-27T11:23:37Z
dc.date.issued2008-07-30
dc.description.abstractTriatomines are of great concern in public health because they are vectors of Chagas' disease. This study presents an analysis of the species Triatoma melanosoma. The cytogenetic characteristics of triatomines include holocentric chromosomes, post-reductional meiosis in the sex chromosomes and nucleolar fragmentation in the meiotic cycle. The methodology utilized consisted of the techniques of lacto-acetic orcein staining and silver ion impregnation. The organs analyzed were adult testicles. The results enabled to classify the chromosomes by number and size, being three large, eight medium and one small heterochromosome. The three largest chromosomes and the heterochromosomes showed heteropyknotic chromatin in meiosis. The heterochromosomes in 8.05% of the cells in metaphase I behaved as pseudobivalents, contrasting with 91.95% of the cells with individualized sex chromosomes, confirming the achiasmatic nature of these chromosomes. However, the pseudobivalents occurred prominently in metaphase II (78.38%), this fact probably is related to the post-reductional nature of the sex chromosomes. The nucleolus in T. melanosoma persisted until the diplotene phase after which it began to fragment. Nucleolar corpuscles were observed in metaphases I and II and during anaphases I and II, these characteristics being related to the phenomenon of nucleolar persistence. In the initial spermatids, peripheral silver ion impregnation occurred, which could be analogous to the pre-nucleolar corpuscles observed after fragmentation. Thus, this study extends our knowledge of the characteristics of triatomines, in particular, heteropyknotic degree, kinetic activity, formation of sex chromosome achiasmatic pseudobivalency, confirmation of the fragmentation phenomenon, and post-meiotic nucleolar reactivation. ©FUNPEC-RP.en
dc.description.affiliationDepartamento de Biologia Instituto de Biociências, Letras e Ciências Exatas Universidade Estadual Paulista Júlio de Mesquita Filho, São José do Rio Preto, SP
dc.description.affiliationUnespDepartamento de Biologia Instituto de Biociências, Letras e Ciências Exatas Universidade Estadual Paulista Júlio de Mesquita Filho, São José do Rio Preto, SP
dc.format.extent326-335
dc.identifierhttp://dx.doi.org/10.4238/vol7-2gmr411
dc.identifier.citationGenetics and Molecular Research, v. 7, n. 2, p. 326-335, 2008.
dc.identifier.doi10.4238/vol7-2gmr411
dc.identifier.file2-s2.0-47949120977.pdf
dc.identifier.issn1676-5680
dc.identifier.lattes6045437265946340
dc.identifier.scopus2-s2.0-47949120977
dc.identifier.urihttp://hdl.handle.net/11449/70496
dc.language.isoeng
dc.relation.ispartofGenetics and Molecular Research
dc.relation.ispartofsjr0,439
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCytogenetics
dc.subjectHolocentric chromosomes
dc.subjectMeiosis
dc.subjectNucleolus
dc.subjectSpermatogenesis
dc.subjectTriatomines
dc.subjectsilver
dc.subjectcell kinetics
dc.subjectcell structure
dc.subjectchromosome number
dc.subjectchromosome size
dc.subjectcytogenetics
dc.subjectmale
dc.subjectmeiosis
dc.subjectmetaphase
dc.subjectnonhuman
dc.subjectnucleolus
dc.subjectparasite vector
dc.subjectsex chromosome
dc.subjectspermatid
dc.subjectspermatogenesis
dc.subjectTriatoma
dc.subjectTriatoma melanosoma
dc.subjecttriatomine
dc.subjectAnimals
dc.subjectChromosomes
dc.subjectCytogenetic Analysis
dc.subjectMale
dc.subjectOxazines
dc.subjectSex Chromosomes
dc.subjectTestis
dc.subjectHeteroptera
dc.subjectReduviidae
dc.titleCytogenetic analysis in the spermatogenesis of Triatoma melanosoma (Reduviidae; Heteroptera)en
dc.typeArtigo
dcterms.licensehttp://www.geneticsmr.com/node/5
unesp.author.lattes6045437265946340
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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