Relationships between cagA, vacA, and iceA genotypes of Helicobacter pylori and DNA damage in the gastric mucosa

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dc.contributor.authorLadeira, MSP
dc.contributor.authorRodrigues, MAM
dc.contributor.authorSalvadori, Daisy Maria Favero [UNESP]
dc.contributor.authorNeto, P. P.
dc.contributor.authorAchilles, P.
dc.contributor.authorLerco, Mauro Masson [UNESP]
dc.contributor.authorRodrigues, Paulo Antônio [UNESP]
dc.contributor.authorGoncalves, I
dc.contributor.authorQueiroz, DMM
dc.contributor.authorFreire-Maia, D. V.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.date.accessioned2014-05-20T13:32:51Z
dc.date.available2014-05-20T13:32:51Z
dc.date.issued2004-01-01
dc.description.abstractHelicobacter pylori (H. pylori) is believed to dispose carriers to gastric cancer by inducing chronic inflammation. The inflammatory processes may result in the generation of reactive oxygen and nitrogen species that damage DNA. In this study, we investigated the relationships between DNA damage in the gastric mucosa and cogA, vocA, and iceA genotypes of H. pylori. The study was conducted with biopsies from the gastric antrum and corpus of 98 H. pylori-infected and 26 uninfected control patients. H. pylori genotypes were determined by PCR and DNA damage was measured in gastric mucosal cells by the Comet assay (single cell gel electrophoresis). All patients were nonsmokers, not abusing alcohol, and not using prescription or recreational drugs. Levels of DNA damage were significantly higher (P < 0.0001) in the H. pylori-infected patients than in uninfected patients. In comparison with the level of DNA damage in the uninfected controls, the extent of DNA damage in both the antrum (OR = 8.45; 95% Cl 2.33-37.72) and the corpus (OR 6.55; 95% Cl 2.52-17.72) was related to infection by cagA(+)/vocAs1m1 and iceA1 strains. The results indicate that the genotype of H. pylori is related to the amount of DNA damage in the gastric mucosa. These genotypes could serve as biomarkers for the risk of extensive DNA damage and possibly gastric cancer. (C) 2004 Wiley-Liss, Inc.en
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Dept Genet, Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Patol, Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Clin Med, Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Med, Dept Cirugia, Botucatu, SP, Brazil
dc.description.affiliationUniv Fed Minas Gerais, Fac Med, Lab Pesquisa Bacteriol, Belo Horizonte, MG, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, Dept Genet, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Patol, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Clin Med, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Med, Dept Cirugia, Botucatu, SP, Brazil
dc.format.extent91-98
dc.identifierhttp://dx.doi.org/10.1002/em.20045
dc.identifier.citationEnvironmental and Molecular Mutagenesis. Hoboken: Wiley-liss, v. 44, n. 2, p. 91-98, 2004.
dc.identifier.doi10.1002/em.20045
dc.identifier.issn0893-6692
dc.identifier.lattes5051118752980903
dc.identifier.lattes2755475227543451
dc.identifier.lattes4728690596167767
dc.identifier.urihttp://hdl.handle.net/11449/11220
dc.identifier.wosWOS:000223211300001
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofEnvironmental and Molecular Mutagenesis
dc.relation.ispartofjcr3.254
dc.relation.ispartofsjr1,119
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectHelicobacter pyloript
dc.subjectgenotypespt
dc.subjectDNA damagept
dc.subjectcomet assaypt
dc.titleRelationships between cagA, vacA, and iceA genotypes of Helicobacter pylori and DNA damage in the gastric mucosaen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.author.lattes5051118752980903
unesp.author.lattes2755475227543451
unesp.author.lattes4728690596167767
unesp.author.orcid0000-0001-9323-3134[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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