Ovarian contrast-enhanced ultrasonography and Doppler fluxometry in bitches during the postovulatory estrus and corpora lutea formation

Página do item simplificado
dc.contributor.authorNogueira Aires, Luiz Paulo [UNESP]
dc.contributor.authorGasser, Beatriz [UNESP]
dc.contributor.authorSilva, Priscila [UNESP]
dc.contributor.authorDel'Aguila-Silva, Priscila [UNESP]
dc.contributor.authorYamada, Diego Iwao
dc.contributor.authorCarneiro, Rafael Kretzer [UNESP]
dc.contributor.authorBruna Bressianini Lima, Bruna [UNESP]
dc.contributor.authorPadilha-Nakaghi, Luciana Cristina [UNESP]
dc.contributor.authorRamirez Uscategui, Ricardo Andrés
dc.contributor.authorSpada, Stefano
dc.contributor.authorRusso, Marco
dc.contributor.authorRossi Feliciano, Marcus Antônio [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionMarilia University (UNIMAR)
dc.contributor.institutionUniversidad CES
dc.contributor.institutionUniversity of Naples “Federico II”
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2023-07-29T13:24:25Z
dc.date.available2023-07-29T13:24:25Z
dc.date.issued2022-12-01
dc.description.abstractThe aim of the present study was to evaluate the vascularization features of canine ovaries during the follicular phase and the formation of the corpora lutea by using Doppler ultrasonography and Contrast-Enhanced Ultrasound (CEUS). Eight healthy bitches were enrolled in the study and were evaluated at five different timepoints (T1 – T5) of the estrous cycle established by vaginal cytology and serum progesterone concentration. Ultrasonographic examinations were performed by a single operator using the ACUSON S2000/SIEMENS machine equipped with a linear multifrequency transducer (9.0 MHz). Color-coded Doppler evaluation of the ovarian parenchyma was performed to investigate the aspects of the signal detection throughout the different timepoints. Pulsed-wave Doppler of the intraovarian arteries was performed to evaluate spectral waveform and doppler velocimetric parameters of Systolic Peak Velocity (SPV cm/s), End Diastolic Velocity (EDV cm/s), Resistivity Index (RI) and Pulsatility Index (PI). CEUS evaluation of the ovaries was performed using a vascular contrast agent (SonoVue®, Bracco, Sao Paulo, Brazil) and the CADENCE™ Contrast Pulse Sequencing (CPS, Siemens) software, in order to perform both qualitative and quantitative analysis. Perfusion parameters of pixel number, peak intensity (PPI in %), time to peak intensity (TTP in s), mean transit time (MTT in s) and area under the curve (AUC in %). Colour-coded Doppler evaluation demonstrated an increase in signal detection as cycle progressed, with blood flow initially detected with few coloured pixels and mainly at the ventral surface of the ovaries. Further on, the number of coloured pixels increased and spreading to the central region, resulting in a circular-like pattern around the corpora hemorrhagica. The spectral waveform was consistent at all timepoints. SPV (cm/s) and EDV (cm/s) presented a numeric trend and a slight statistical difference at all timepoints, whereas no difference was found for RI and PI. CEUS evaluation demonstrated an increase in pixel intensity across all the timepoints. Quantitative CEUS analysis revealed a statistical difference in PPI (%), MTT (s) and AUC (%) at T5. CEUS evaluation of the ovaries was feasible and demonstrated a marked increase in perfusion parameters in the late postovulatory period, demonstrating its applicability in the assessment of canine corpora lutea development.en
dc.description.affiliationDepartment of Pathology Reproduction and One Health School of Agricultural and Veterinarian Sciences Sao Paulo State University “Julio de Mesquita Filho” (FCAV/UNESP), Sao Paulo
dc.description.affiliationDepartment of Veterinary Clinic and Surgery School of Agricultural and Veterinarian Sciences Sao Paulo State University “Júlio de Mesquita Filho” (FCAV/UNESP), Sao Paulo
dc.description.affiliationMarilia University (UNIMAR), Sao Paulo
dc.description.affiliationResearch Group INCA-CES Faculty of Veterinary Medicine and Animal Science Universidad CES
dc.description.affiliationDepartment of Veterinary Medicine and Animal Productions University of Naples “Federico II”
dc.description.affiliationLaboratory of Veterinary Imaginology Faculty of Animal Science and Food Engineering (FZEA) Sao Paulo University (USP), Sao Paulo
dc.description.affiliationUnespDepartment of Pathology Reproduction and One Health School of Agricultural and Veterinarian Sciences Sao Paulo State University “Julio de Mesquita Filho” (FCAV/UNESP), Sao Paulo
dc.description.affiliationUnespDepartment of Veterinary Clinic and Surgery School of Agricultural and Veterinarian Sciences Sao Paulo State University “Júlio de Mesquita Filho” (FCAV/UNESP), Sao Paulo
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCNPq: nº 130820/2019-9
dc.description.sponsorshipIdFAPESP: nº 2017/14957-6
dc.description.sponsorshipIdFAPESP: nº 2019/15282-8
dc.description.sponsorshipIdCNPq: nº 309199/2017-4
dc.description.sponsorshipIdCNPq: nº 430023/2018-9
dc.format.extent162-170
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2022.10.009
dc.identifier.citationTheriogenology, v. 194, p. 162-170.
dc.identifier.doi10.1016/j.theriogenology.2022.10.009
dc.identifier.issn0093-691X
dc.identifier.scopus2-s2.0-85139831028
dc.identifier.urihttp://hdl.handle.net/11449/247738
dc.language.isoeng
dc.relation.ispartofTheriogenology
dc.sourceScopus
dc.subjectCanine. ovarian perfusion. ovarian follicle. corpus luteum. angiogenesis
dc.titleOvarian contrast-enhanced ultrasonography and Doppler fluxometry in bitches during the postovulatory estrus and corpora lutea formationen
dc.typeArtigo
unesp.author.orcid0000-0003-4373-6539 0000-0003-4373-6539 0000-0003-4373-6539[12]

Arquivos

Coleções

Artigos - Patologia Veterinária - FCAV
Artigos - Clínica e Cirurgia Veterinária - FCAV