Recellularized rat testis scaffolds with embryoid bodies cells: a promising approach for tissue engineering

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dc.contributor.authorBatista, Vitória Frias
dc.contributor.authorde Sá Schiavo Matias, Gustavo
dc.contributor.authorCarreira, Ana Claudia Oliveira
dc.contributor.authorSmith, Lawrence Charles
dc.contributor.authorRodrigues, Rafaela
dc.contributor.authorAraujo, Michelle Silva
dc.contributor.authorSouza Silva, Dara Rubia
dc.contributor.authorMoraes, Felipe de Jesus
dc.contributor.authorGarcia, Joaquim Mansano [UNESP]
dc.contributor.authorMiglino, Maria Angelica
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversité de Montréal)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-05-01T13:41:28Z
dc.date.available2022-05-01T13:41:28Z
dc.date.issued2022-01-01
dc.description.abstractTissue engineering is gaining use to investigate the application of its techniques for infertility treatment. The use of pluripotent embryonic cells for in vitro production of viable spermatozoa in testicular scaffolds is a promising strategy that could solve male infertility. Due to cell–extracellular matrix (ECM) interactions, here we aim to investigate the differentiation of embryoid bodies (EBs) in cultured into decellularized rat testis scaffolds. Decellularized testis (P = 0.019) with a low concentration of gDNA (30.58 mg/ng tissue) was obtained by sodium dodecyl sulfate perfusion. The structural proteins (collagens type I and III) and the adhesive glycoproteins of ECM (laminin and fibronectin) were preserved according to histological and scanning electron microscopy (SEM) analyses. Then, decellularized rat testis were cultured for 7 days with EB, and EB mixed with retinoic acid (RA) in non-adherent plates. By SEM, we observe that embryonic stem cells adhered in the decellularized testis ECM. By immunofluorescence, we verified the positive expression of HSD17B3, GDNF, ACRV-1, and TRIM-36, indicating their differentiation using RA in vitro, reinforcing the possibility of EB in male germ cell differentiation. Finally, recellularized testis ECM may be a promising tool for future new approaches for testicular cell differentiation applied to assisted reproduction techniques and infertility treatment. Abbreviations: ACRV-1: Acrosomal vesicle protein 1; ATB: Penicillin-streptomycin; DAPI: 4,6-Diamidino-2-phenylindole; EB: Embryoid bodies; ECM: Extracellular matrix; ESCs: Pluripotent embryonic stem cells; GAGs: Glycosaminoglycans; gDNA: Genomic DNA; GDNF: Glial cell line-derived neurotrophic factor; H&E: Hematoxylin and eosin; HSD17B3: 17-beta-Hydroxysteroid dehydrogenase type 3; PBS: Phosphate-buffered saline; PGCLCs: Primordial germ-cell-like cells; RA: Retinoic acid; SDS: Sodium dodecyl sulfate; SEM: Scanning electron microscopy; SSCs: Spermatogonial stem cells; TRIM-36: Tripartite Motif Containing 36;.en
dc.description.affiliationDepartment of Surgery School of Veterinary Medicine and Animal Science University of São Paulo
dc.description.affiliationCentre de Recherche En Reproduction Et Fertilité Université de Montréal)
dc.description.affiliationDepartment of Preventive Veterinary Medicine and Animal Reproduction (Reproduction) São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Preventive Veterinary Medicine and Animal Reproduction (Reproduction) São Paulo State University (UNESP)
dc.format.extent44-54
dc.identifierhttp://dx.doi.org/10.1080/19396368.2021.2007554
dc.identifier.citationSystems Biology in Reproductive Medicine, v. 68, n. 1, p. 44-54, 2022.
dc.identifier.doi10.1080/19396368.2021.2007554
dc.identifier.issn1939-6376
dc.identifier.issn1939-6368
dc.identifier.scopus2-s2.0-85124084360
dc.identifier.urihttp://hdl.handle.net/11449/234103
dc.language.isoeng
dc.relation.ispartofSystems Biology in Reproductive Medicine
dc.sourceScopus
dc.subjectDecellularized testicular matrix
dc.subjectembryonic stem cells
dc.subjectmale infertility
dc.subjectreproduction
dc.titleRecellularized rat testis scaffolds with embryoid bodies cells: a promising approach for tissue engineeringen
dc.typeArtigo

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Artigos - Medicina Veterinária Preventiva e Reprodução Animal - FCAV