Immunodiagnosis of swine cysticercosis by indirect ELISA employing a heterologous antigen from Taenia crassiceps metacestode

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dc.contributor.authorBiondi, Germano Francisco [UNESP]
dc.contributor.authorMucciolo, R. G.
dc.contributor.authorNunes, Caris Maroni [UNESP]
dc.contributor.authorRichtzenhain, L. J.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T15:22:06Z
dc.date.available2014-05-20T15:22:06Z
dc.date.issued1996-09-16
dc.description.abstractC. Larralde et al. (1990, Arch. Pathol. Lab. Med., 114:926-928) demonstrated that heterologous antigen from the laboratory-adapted murine Taenia crassiceps metacestode may substitute those from Taenia solium in the immunodiagnosis of human cysticercosis by the indirect enzyme-linked immunosorbent assay (IE). ?This antigen is easily obtained at a laboratory level and solves the problem of T. solium cysticerci collection from naturally or experimentally infected swine. In this study an IE employing a heterologous antigen from the T. crassiceps metacestode was evaluated for the immunodiagnosis of swine cysticercosis. Sera from 300 swine free of T. solium cysticerci by post-mortem examination were employed to determine two IE cut- off values: 1) Mean ELISA values + 2 standard deviations (2 sigma cut-off) and 2) - Mean ELISA values + 3 standard deviations (3 sigma cut-off). The specificity of IE was 97% with the 2 sigma cut-off and 100% with the 3 sigma cut-off. When applied to ten sera from swine infected by cysticerci of T. solium by post-mortem examination, the sensitivity of IE was 100% independent of the cut off.en
dc.description.affiliationSAO PAULO STATE UNIV,FAC VET MED,DEPT VET HYG & PUBL HLTH,BOTUCATU,SP,BRAZIL
dc.description.affiliationUNIV SAO PAULO,DEPT ANIM REPROD,BR-05508 SAO PAULO,BRAZIL
dc.description.affiliationSAO PAULO STATE UNIV,DEPT ANIM HLTH & PROD,ARACATUBA,BRAZIL
dc.description.affiliationUNIV SAO PAULO,DEPT PREVENT VET MED & ANIM HLTH,BR-05508 SAO PAULO,BRAZIL
dc.description.affiliationUnespSAO PAULO STATE UNIV,FAC VET MED,DEPT VET HYG & PUBL HLTH,BOTUCATU,SP,BRAZIL
dc.description.affiliationUnespSAO PAULO STATE UNIV,DEPT ANIM HLTH & PROD,ARACATUBA,BRAZIL
dc.format.extent261-266
dc.identifierhttp://dx.doi.org/10.1016/0304-4017(95)00928-0
dc.identifier.citationVeterinary Parasitology. Amsterdam: Elsevier B.V., v. 64, n. 4, p. 261-266, 1996.
dc.identifier.doi10.1016/0304-4017(95)00928-0
dc.identifier.issn0304-4017
dc.identifier.lattes1892359871207408
dc.identifier.urihttp://hdl.handle.net/11449/33165
dc.identifier.wosWOS:A1996VN48300001
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofVeterinary Parasitology
dc.relation.ispartofjcr2.422
dc.relation.ispartofsjr1,275
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectswine cysticercosispt
dc.subjectimmunodiagnosispt
dc.subjectindirect ELISApt
dc.subjectheterologous antigenpt
dc.subjectTaenia crassicepspt
dc.titleImmunodiagnosis of swine cysticercosis by indirect ELISA employing a heterologous antigen from Taenia crassiceps metacestodeen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes8759101165889150
unesp.author.lattes1892359871207408[3]
unesp.author.orcid0000-0002-5463-3845[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatubapt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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