Insulin-like growth factor-1 (IGF-1) selectively modulates the metabolic and lipid profile of bovine embryos according to their kinetics of development

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dc.contributor.authorAnnes, Kelly
dc.contributor.authorde Lima, Camila Bruna
dc.contributor.authorIspada, Jéssica
dc.contributor.authordos Santos, Érika Cristina
dc.contributor.authorFontes, Patrícia Kubo
dc.contributor.authorNichi, Marcílio
dc.contributor.authorNogueira, Marcelo Fabio Gouveia [UNESP]
dc.contributor.authorSudano, Mateus José
dc.contributor.authorMilazzotto, Marcella Pecora
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniversité Laval
dc.contributor.institutionFederal University of ABC
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T13:49:04Z
dc.date.available2023-07-29T13:49:04Z
dc.date.issued2023-07-01
dc.description.abstractSupplementation of culture media with IGF-1 during in vitro culture of embryos has had controversial results over the years. In the present study, we show that differences previously observed in response to IGF addition might be related to intrinsic heterogeneity of the embryos. In other words, the effects exerted by IGF-1 are dependent on the characteristics of the embryos and their ability to modulate metabolism and overcome stressful conditions, such as the ones found in a non-optimized in vitro culture system. To test this hypothesis, in vitro produced bovine embryos with distinct morphokinetics (fast- and slow-cleavage) were submitted to treatment with IGF-1 and then evaluated for embryo production rates, total cell number, gene expression and lipid profile. Our results show that remarkable differences were found when fast and slow embryos treated with IGF-1 were compared. Fast embryos respond by upregulating genes related to mitochondrial function, stress response, and lipid metabolism, whereas slow embryos presented lower mitochondrial efficiency and lipid accumulation. We conclude that indeed the treatment with IGF-1 selectively affects embryonic metabolism according to early morphokinetics phenotypes, and this information is relevant for decision-making in the design of more appropriate in vitro culture systems.en
dc.description.affiliationDepartment of Genetics and Evolution Federal University of São Carlos, SP
dc.description.affiliationCentre de Recherche en Reproduction Développement et Santé Intergénérationnelle (CRDSI) Département des Sciences Animales Université Laval
dc.description.affiliationLaboratory of Embryonic Metabolism and Epigenetic Center of Natural and Human Science Federal University of ABC, SP
dc.description.affiliationDepartment of Animal Reproduction Faculty of Veterinary Medicine and Animal Science University of São Paulo, SP
dc.description.affiliationDepartment of Biological Sciences School of Sciences and Languages São Paulo State University, Campus Assis, SP
dc.description.affiliationUnespDepartment of Biological Sciences School of Sciences and Languages São Paulo State University, Campus Assis, SP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: #16/05986-0
dc.description.sponsorshipIdFAPESP: #17/18384-0
dc.format.extent1-7
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2023.03.020
dc.identifier.citationTheriogenology, v. 204, p. 1-7.
dc.identifier.doi10.1016/j.theriogenology.2023.03.020
dc.identifier.issn0093-691X
dc.identifier.scopus2-s2.0-85151504203
dc.identifier.urihttp://hdl.handle.net/11449/248621
dc.language.isoeng
dc.relation.ispartofTheriogenology
dc.sourceScopus
dc.subjectBovine
dc.subjectEmbryos
dc.subjectIGF
dc.subjectLipids
dc.subjectMorphokinetics
dc.titleInsulin-like growth factor-1 (IGF-1) selectively modulates the metabolic and lipid profile of bovine embryos according to their kinetics of developmenten
dc.typeArtigo
unesp.author.orcid0000-0003-0933-3066[9]

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