Genetic and Antimicrobial Resistance Profiles of Mammary Pathogenic E. coli (MPEC) Isolates from Bovine Clinical Mastitis

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dc.contributor.authorCampos, Fernanda C. [UNESP]
dc.contributor.authorCastilho, Ivana G. [UNESP]
dc.contributor.authorRossi, Bruna F. [UNESP]
dc.contributor.authorBonsaglia, Érika C. R. [UNESP]
dc.contributor.authorDantas, Stéfani T. A. [UNESP]
dc.contributor.authorDias, Regiane C. B. [UNESP]
dc.contributor.authorFernandes Júnior, Ary [UNESP]
dc.contributor.authorHernandes, Rodrigo T. [UNESP]
dc.contributor.authorCamargo, Carlos H.
dc.contributor.authorRibeiro, Márcio G. [UNESP]
dc.contributor.authorPantoja, José C. F. [UNESP]
dc.contributor.authorLangoni, Hélio [UNESP]
dc.contributor.authorRall, Vera L. M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionAdolfo Lutz Institute
dc.date.accessioned2023-07-29T13:34:05Z
dc.date.available2023-07-29T13:34:05Z
dc.date.issued2022-12-01
dc.description.abstractMammary pathogenic E. coli (MPEC) is one of the main pathogens of environmental origin responsible for causing clinical mastitis worldwide. Even though E. coli are strongly associated with transient or persistent mastitis and the economic impacts of this disease, the virulence factors involved in the pathogenesis of MPEC remain unknown. Our aim was to characterize 110 MPEC isolates obtained from the milk of cows with clinical mastitis, regarding the virulence factor-encoding genes present, adherence patterns on HeLa cells, and antimicrobial resistance profile. The MPEC isolates were classified mainly in phylogroups A (50.9%) and B1 (38.2%). None of the isolates harbored genes used for diarrheagenic E. coli classification, but 26 (23.6%) and 4 (3.6%) isolates produced the aggregative or diffuse adherence pattern, respectively. Among the 22 genes investigated, encoding virulence factors associated with extraintestinal pathogenic E. coli pathogenesis, fimH (93.6%) was the most frequent, followed by traT (77.3%) and ompT (68.2%). Pulsed-field gel electrophoresis analysis revealed six pulse-types with isolates obtained over time, thus indicating persistent intramammary infections. The genes encoding beta-lactamases detected were as follows: blaTEM (35/31.8%); blaCTX-M-2/blaCTX-M-8 (2/1.8%); blaCTX-M-15 and blaCMY-2 (1/0.9%); five isolates were classified as extended spectrum beta-lactamase (ESBL) producers. As far as we know, papA, shf, ireA, sat and blaCTX-M-8 were detected for the first time in MPEC. In summary, the genetic profile of the MPEC studied was highly heterogeneous, making it impossible to establish a common genetic profile useful for molecular MPEC classification. Moreover, the detection of ESBL-producing isolates is a serious public health concern.en
dc.description.affiliationDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University
dc.description.affiliationBacteriology Division Adolfo Lutz Institute
dc.description.affiliationDepartment of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Sciences São Paulo State University
dc.description.affiliationUnespDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University
dc.description.affiliationUnespDepartment of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Sciences São Paulo State University
dc.identifierhttp://dx.doi.org/10.3390/pathogens11121435
dc.identifier.citationPathogens, v. 11, n. 12, 2022.
dc.identifier.doi10.3390/pathogens11121435
dc.identifier.issn2076-0817
dc.identifier.scopus2-s2.0-85144714460
dc.identifier.urihttp://hdl.handle.net/11449/248087
dc.language.isoeng
dc.relation.ispartofPathogens
dc.sourceScopus
dc.subjectESBL
dc.subjectintramammary infection
dc.subjectMPEC
dc.subjectphylogroup
dc.subjectvirulence
dc.titleGenetic and Antimicrobial Resistance Profiles of Mammary Pathogenic E. coli (MPEC) Isolates from Bovine Clinical Mastitisen
dc.typeArtigo
unesp.author.orcid0000-0001-7839-4677[5]
unesp.author.orcid0000-0001-6695-6003[8]
unesp.author.orcid0000-0001-5127-0762[12]
unesp.author.orcid0000-0003-4280-5619[13]

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