Anaerobic microorganisms in root canals of human teeth with chronic apical periodontitis detected by indirect immunofluorescence

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dc.contributor.authorAssed, S.
dc.contributor.authorIto, I. Y.
dc.contributor.authorLeonardo, M. R. [UNESP]
dc.contributor.authorSilva, L. A B
dc.contributor.authorLopatin, D. E.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Michigan
dc.date.accessioned2014-05-27T11:18:04Z
dc.date.available2014-05-27T11:18:04Z
dc.date.issued1996-04-01
dc.description.abstractAiming to assess the presence of selected anaerobic microorganisms in root canals of human teeth with chronic apical periodontitis, 25 central and lateral upper incisors presenting with radiographic evidence of chronic apical periodontitis were studied. The pulp chamber was opened under aseptic conditions and samples of the root canal content were collected with sterile absorbent paper points, which were placed and dispersed in test tubes containing reduced transport medium (RTF). Aliquots were dried on glass slides and stained by indirect immunofluorcscencc for detection of Actinomyces viscosus, Fusobacterium nucleatum, Porphyromonas gingivalis and Prevotella intermedia. The results showed a positive indirect immunofluorescence reaction in 24 of the 25 samples. Fourteen were positive for the specie Actinomyces viscosus, 12 for Prevotella intermedia, 10 for Fusobacterium nucleatum and 4 for Porphyromonas gingivalis. A semiquantitative assay was easily implemented for assessment of degree of infection by the organisms in individual cases. © Munksgaard, 1996.en
dc.description.affiliationDepartment of Pediatric Dentistry Ribeirão Preto School of Pharmacy USP
dc.description.affiliationDepartment of Health Sciences Ribeirão Preto School of Pharmacy USP
dc.description.affiliationDepartment of Endodontics Araraquara School of Dentistry UNESP
dc.description.affiliationDepartment of Biologic and Materials Sciences School of Dentistry University of Michigan, Ann Arbor, MI
dc.description.affiliationDepartment of Endodontics Faculdade de Odontologia de Araraquara UNESP, P.O. Box 331, 14801-385, Araraquara, SP
dc.description.affiliationUnespDepartment of Endodontics Araraquara School of Dentistry UNESP
dc.description.affiliationUnespDepartment of Endodontics Faculdade de Odontologia de Araraquara UNESP, P.O. Box 331, 14801-385, Araraquara, SP
dc.format.extent66-69
dc.identifierhttp://dx.doi.org/10.1111/j.1600-9657.1996.tb00099.x
dc.identifier.citationEndodontics and Dental Traumatology, v. 12, n. 2, p. 66-69, 1996.
dc.identifier.doi10.1111/j.1600-9657.1996.tb00099.x
dc.identifier.issn0109-2502
dc.identifier.scopus2-s2.0-0030113305
dc.identifier.urihttp://hdl.handle.net/11449/64770
dc.language.isoeng
dc.relation.ispartofEndodontics and Dental Traumatology
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAnaerobic microorganisms
dc.subjectApical periodontitis
dc.subjectIndirect immunofluorescence
dc.titleAnaerobic microorganisms in root canals of human teeth with chronic apical periodontitis detected by indirect immunofluorescenceen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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