Encapsulation of entomopathogenic fungal conidia: evaluation of stability and control potential of Rhipicephalus microplus

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dc.contributor.authorMeirelles, Laura Nobrega
dc.contributor.authorMesquita, Emily
dc.contributor.authorCorrêa, Thaís Almeida
dc.contributor.authorBitencourt, Ricardo de Oliveira Barbosa
dc.contributor.authorOliveira, Jhones Luiz [UNESP]
dc.contributor.authorFraceto, Leonardo Fernandes [UNESP]
dc.contributor.authorCamargo, Mariana Guedes
dc.contributor.authorBittencourt, Vânia Rita Elias Pinheiro
dc.contributor.institutionFederal Rural University of Rio de Janeiro
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T13:52:18Z
dc.date.available2023-07-29T13:52:18Z
dc.date.issued2023-07-01
dc.description.abstractThe use of chemical acaricides is the primary strategy to control tick infestations. Nonetheless, chemical resistance in ticks has been reported. Thus, complementary methods such as biological control using entomopathogenic fungi (EPF) have been investigated. EPF, although efficient, have their viability compromised when applied under natural conditions, which indicates that formulation development is essential. Some researchers have demonstrated the efficacy of ionic gelation in protecting EPF against deleterious abiotic factors. In the present study, we conducted the ionic gelation technique to encapsulate Metarhizium anisopliae (Metschn.) Sorokin (Hypocreales: Clavicipitaceae) conidia in 2% (EC 2%) and 3% (EC 3%) sodium alginate. Next, the quantity and viability of encapsulated conidia (EC) were determined. The morphology of particles was characterized by using Scanning Electron Microscopy (SEM). EC and non-encapsulated conidia (NEC) were stored at room temperature (26.8 °C) and in the freezer (-11.9 °C) to shelf-life testing. For UV-B irradiance tolerance and thermotolerance tests, EC and NEC were exposed to UV-B (6.0 or 8.0 kJ m − 2) and heat (42 ºC). In addition, biological parameters of Rhipicephalus microplus Canestrini (Acari: Ixodidae) engorged females exposed to EC were evaluated. The particles presented a spherical shape, more homogeneous (EC 2%) or heterogeneous (EC 3%). Encapsulation decreased (4.8×) the conidial concentration and did not affect their viability. On the other hand, encapsulation increased the shelf life of conidia at room temperature as well as their UV-B tolerance and thermotolerance (6 h). The fungal particles decreased the biological parameters of females more significantly than the NEC. As far as we know, we reported for the first time the use of the ionic gelation to encapsulate entomopathogenic fungi toward controlling R. microplus.en
dc.description.affiliationProgram in Veterinary Sciences Veterinary Institute Federal Rural University of Rio de Janeiro, Rodovia BR 465, s/n, RJ
dc.description.affiliationEnvironmental Nanotechnology Laboratory Institute of Science and Technology of Sorocaba São Paulo State University, Av. Três de Março, 511 - Alto da Boa Vista, SP
dc.description.affiliationDepartment of Animal Parasitology Veterinary Institute Federal Rural University of Rio de Janeiro, Rodovia BR 465, s/n, RJ
dc.description.affiliationUnespEnvironmental Nanotechnology Laboratory Institute of Science and Technology of Sorocaba São Paulo State University, Av. Três de Março, 511 - Alto da Boa Vista, SP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
dc.description.sponsorshipIdFAPESP: 2018/21142-1
dc.description.sponsorshipIdCNPq: CNPq - 308439/2021-0
dc.description.sponsorshipIdFAPERJ: E-26/202.957/2017
dc.description.sponsorshipIdFAPESP: FAPESP #2017/21004-5
dc.description.sponsorshipIdCNPq: Project 302985/2019-0
dc.identifierhttp://dx.doi.org/10.1016/j.ttbdis.2023.102184
dc.identifier.citationTicks and Tick-borne Diseases, v. 14, n. 4, 2023.
dc.identifier.doi10.1016/j.ttbdis.2023.102184
dc.identifier.issn1877-9603
dc.identifier.issn1877-959X
dc.identifier.scopus2-s2.0-85153492757
dc.identifier.urihttp://hdl.handle.net/11449/248738
dc.language.isoeng
dc.relation.ispartofTicks and Tick-borne Diseases
dc.sourceScopus
dc.subjectBiological control
dc.subjectCattle tick
dc.subjectIonic gelation
dc.subjectMetarhizium anisopliae
dc.subjectSodium alginate
dc.titleEncapsulation of entomopathogenic fungal conidia: evaluation of stability and control potential of Rhipicephalus microplusen
dc.typeArtigo
unesp.author.orcid0000-0002-2214-5038[1]
unesp.author.orcid0000-0002-4263-1332[2]
unesp.author.orcid0000-0001-6909-2925[3]
unesp.author.orcid0000-0002-6298-2869[4]
unesp.author.orcid0000-0003-4132-8910[7]
unesp.author.orcid0000-0001-8473-8501[8]

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