Avaliação da eficácia da solução ozonizada à base de D-Pantenol, Prata Nanoparticulada, Ácido Salicílico e Óleos Essenciais no tratamento de feridas cutâneas em Rattus norvegicus (Wistar)
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Data
2023-03-02
Autores
Sfrizo, Luciana de Simone
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Universidade Estadual Paulista (Unesp)
Resumo
Feridas cutâneas representam alta prevalência no atendimento clínico de pequenos animais. No entanto, seu tratamento é um desafio comum, pois, em muitos casos, possuem manejo de longo prazo, o que pode gerar grande morbidade aos pacientes e desgastes aos tutores. Há diversidade considerável de produtos disponíveis no mercado, os quais buscam redução no tempo do processo cicatricial. Entretanto muitos deles possuem custos elevados e são por vezes até mesmo tóxicos à longo prazo. Objetivou-se com este estudo, avaliar uma opção terapêutica, administrada por via tópica, por meio da combinação de sete compostos naturais ozonizados a base de D-Pantenol, Prata Nanoparticulada, Ácido Salicílico e Óleos Essenciais (tomilho, Aloe vera, alecrim e malva), para a cicatrização por segunda intenção de feridas cutâneas em ratos (Rattus norvegicus). Para isso, foram utilizados 30 ratos, distribuídos aleatoriamente no grupo controle (GC), o qual seria administrado solução salina 0,9%, e grupo tratado (GT) em que seria administrado solução ozonizada à base D-Pantenol, Prata Nanoparticulada, Ácido Salicílico e Óleos Essenciais (tomilho, Aloe vera, alecrim e malva). Foram confeccionados dois defeitos na região dorsal de cada animal, em que no defeito cranial foi utilizado para avaliação da evolução da cicatrização por meio de planimetria digital, utilizando o “Software ImageJ”, e o defeito caudal para avaliação macroscópica da presença de sinais de inflamação, necrose, edema, crosta, tecido de granulação, secreção, aspecto da secreção, coloração da ferida e aderência. As avaliações e a administração das soluções ocorreram em momentos pré-determinados T3 (três dias de pós-operatório), T7 (sete dias de pós-operatório) e T14 (quatorze dias de pós-operatório). Em relação à mensuração da área (cm²) da ferida, foi observado diferença significativa entre os grupos no terceiro dia de avaliação, em que o grupo tratado apresentou maior área. Quanto à diferença de área nos diferentes momentos de avaliação, foi constatada diferença significativa do grupo controle (T3 – T7), em relação às outras diferenças de área (T7-T14; T3-T14) dentro deste grupo e quando comparado com o grupo tratado. Quando analisados os resultados obtidos na avaliação macroscópica, observou-se que houve diferença significativa em relação às variáveis inflamação, necrose e tecido de granulação, em que o grupo tratado apresentou no terceiro dia de experimento número maior de animais com inflamação, necrose e tecido de granulação quando comparado com o grupo controle. Tanto a solução salina 0,9% quanto a solução ozonizada contribuíram para a cicatrização das feridas, fazendo-se assim, necessários mais estudos em relação à dose/efeito da solução ozonizada.
Skin wounds represent a high prevalence in the small animals clinical care. However, their treatment is a common challenge, since in many cases, they have long-term management, which can result in great morbidity for patients and distress for clients. There is a considerable diversity of products available, which attempt to reduce the time of the healing process. However many of them have costs and are sometimes even toxic in long term use. The aim of this study was to evaluate the administration of a therapeutic option, administered topically, through the combination of seven natural ozonated compounds based on D-Panthenol, Nanoparticulated Silver, Salicylic Acid and Essential Oils (thyme, Aloe vera, rosemary and malva), for the healing by second intention of skin wounds in rats (Rattus norvegicus). For this, thirty rats were used, randomly distributed in the control group (GC), which would be administered saline solution 0,9%, and treated group (TG) in which ozonated solution based on D-Panthenol, Nanoparticulated Silver, Salicylic Acid and Essential Oils (thyme, Aloe vera, rosemary and malva). Two defects were made in the cutaneous dorsal region of each animal, in which the cranial defect was used to assess the evolution of healing through digital planimetry, using the “ImageJ Software” and the caudal defect for macroscopic evaluation of the presence of sings of inflammation, necrosis, edema, crust, granulation tissue, secretion, appearance of the secretion, wound color and adherence. The evaluations and administration of the solutions occurred at predetermined moments T3 (three days after the surgery), T7 (seven days after the surgery) and T14 (fourteen days after the surgery). Regarding the measurement of the area (cm²) from de wound, a significant difference was observed between the groups on the third day of evaluation, in which the treated group had a larger area. As for the area difference at different evaluation times, a significant difference was found in the control group (T3 - T7), in compare to other area differences (T7-T14; T3-T14) within this group and when compared with the treated group. When analyzing the results obtained in the macroscopic evaluation, it was observed that there was a significant difference in variables of inflammation, necrosis and granulation tissue, in which the treated group presented, on the third day of the experiment, greater number of animals with inflammation, necrosis and granulation tissue when compared with the control group. Both the 0.9% saline solution and the ozonized solution contributed to the healing of wounds, thus making further studies necessary in relation to the dose/effect of the ozonated solution.
Skin wounds represent a high prevalence in the small animals clinical care. However, their treatment is a common challenge, since in many cases, they have long-term management, which can result in great morbidity for patients and distress for clients. There is a considerable diversity of products available, which attempt to reduce the time of the healing process. However many of them have costs and are sometimes even toxic in long term use. The aim of this study was to evaluate the administration of a therapeutic option, administered topically, through the combination of seven natural ozonated compounds based on D-Panthenol, Nanoparticulated Silver, Salicylic Acid and Essential Oils (thyme, Aloe vera, rosemary and malva), for the healing by second intention of skin wounds in rats (Rattus norvegicus). For this, thirty rats were used, randomly distributed in the control group (GC), which would be administered saline solution 0,9%, and treated group (TG) in which ozonated solution based on D-Panthenol, Nanoparticulated Silver, Salicylic Acid and Essential Oils (thyme, Aloe vera, rosemary and malva). Two defects were made in the cutaneous dorsal region of each animal, in which the cranial defect was used to assess the evolution of healing through digital planimetry, using the “ImageJ Software” and the caudal defect for macroscopic evaluation of the presence of sings of inflammation, necrosis, edema, crust, granulation tissue, secretion, appearance of the secretion, wound color and adherence. The evaluations and administration of the solutions occurred at predetermined moments T3 (three days after the surgery), T7 (seven days after the surgery) and T14 (fourteen days after the surgery). Regarding the measurement of the area (cm²) from de wound, a significant difference was observed between the groups on the third day of evaluation, in which the treated group had a larger area. As for the area difference at different evaluation times, a significant difference was found in the control group (T3 - T7), in compare to other area differences (T7-T14; T3-T14) within this group and when compared with the treated group. When analyzing the results obtained in the macroscopic evaluation, it was observed that there was a significant difference in variables of inflammation, necrosis and granulation tissue, in which the treated group presented, on the third day of the experiment, greater number of animals with inflammation, necrosis and granulation tissue when compared with the control group. Both the 0.9% saline solution and the ozonized solution contributed to the healing of wounds, thus making further studies necessary in relation to the dose/effect of the ozonated solution.
Descrição
Palavras-chave
Fitoterápico, Lesões cutâneas, Veterinária de pequenos animais, Cicatrização de feridas