Leptin into the ventrolateral medulla facilitates chemorespiratory response in leptin-deficient (ob/ob) mice

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dc.contributor.authorBassi, M. [UNESP]
dc.contributor.authorFuruya, W. I. [UNESP]
dc.contributor.authorMenani, José Vanderlei [UNESP]
dc.contributor.authorColombari, Débora Simões de Almeida [UNESP]
dc.contributor.authorCarmo, J. M. do
dc.contributor.authorSilva, A. A. da
dc.contributor.authorHall, J. E.
dc.contributor.authorMoreira, T. S.
dc.contributor.authorWenker, I. C.
dc.contributor.authorMulkey, D. K.
dc.contributor.authorColombari, Eduardo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Mississippi
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniv Connecticut
dc.date.accessioned2014-12-03T13:10:48Z
dc.date.available2014-12-03T13:10:48Z
dc.date.issued2014-05-01
dc.description.abstractAimLeptin, an adipocyte-derived hormone, is suggested to participate in the central control of breathing. We hypothesized that leptin may facilitate ventilatory responses to chemoreflex activation by acting on respiratory nuclei of the ventrolateral medulla. The baseline ventilation and the ventilatory responses to CO2 were evaluated before and after daily injections of leptin into the retrotrapezoid nucleus/parafacial respiratory group (RTN/pFRG) for 3days in obese leptin-deficient (ob/ob) mice.MethodsMale ob/ob mice (40-45g, n=7 per group) received daily microinjections of vehicle or leptin (1g per 100nL) for 3days into the RTN/pFRG. Respiratory responses to CO2 were measured by whole-body plethysmography.ResultsUnilateral microinjection of leptin into the RTN/pFRG in ob/ob mice increased baseline ventilation (V-E) from 144796 to 2405 +/- 174mLmin(-1)kg(-1) by increasing tidal volume (V-T) from 6.4 +/- 0.4 to 9.1 +/- 0.8mLkg(-1) (P<0.05). Leptin also enhanced ventilatory responses to 7% CO2 (=2172 +/- 218mLmin(-1)kg(-1), vs. control: =1255 +/- 105mLmin(-1)kg(-1)), which was also due to increased V-T (=4.71 +/- 0.51mLkg(-1), vs. control: =2.27 +/- 0.20mLkg(-1)), without changes in respiratory frequency. Leptin treatment into the RTN/pFRG or into the surrounding areas decreased food intake (83 and 70%, respectively), without significantly changing body weight.ConclusionThe present results suggest that leptin acting in the respiratory nuclei of the ventrolateral medulla improves baseline V-E and V-T and facilitates respiratory responses to hypercapnia in ob/ob mice.en
dc.description.affiliationSao Paulo State Univ UNESP, Sch Dent, Dept Physiol & Pathol, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUniv Mississippi, Med Ctr, Dept Physiol & Biophys, Jackson, MS 39216 USA
dc.description.affiliationUniv Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-09500900 Sao Paulo, Brazil
dc.description.affiliationUniv Connecticut, Dept Physiol & Neurobiol, Storrs, CT 06269 USA
dc.description.affiliationUnespSao Paulo State Univ UNESP, Sch Dent, Dept Physiol & Pathol, BR-14801903 Araraquara, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipNational Heart, Lung and Blood Institute grant
dc.description.sponsorshipIdNational Heart, Lung and Blood Institute grantPO1 HL 51971
dc.description.sponsorshipIdNational Heart, Lung and Blood Institute grantHL104101
dc.format.extent240-248
dc.identifierhttp://dx.doi.org/10.1111/apha.12257
dc.identifier.citationActa Physiologica. Hoboken: Wiley-blackwell, v. 211, n. 1, p. 240-248, 2014.
dc.identifier.doi10.1111/apha.12257
dc.identifier.issn1748-1708
dc.identifier.lattes1023597870118105
dc.identifier.lattes4544450092427426
dc.identifier.urihttp://hdl.handle.net/11449/112525
dc.identifier.wosWOS:000334488200024
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofActa Physiologica
dc.relation.ispartofjcr5.930
dc.relation.ispartofsjr1,542
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectbreathingen
dc.subjectcentral chemoreceptionen
dc.subjectleptinen
dc.subjectobesityen
dc.subjectventrolateral medullaen
dc.titleLeptin into the ventrolateral medulla facilitates chemorespiratory response in leptin-deficient (ob/ob) miceen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.author.lattes1023597870118105
unesp.author.lattes4544450092427426[11]
unesp.author.orcid0000-0003-1167-4441[3]
unesp.author.orcid0000-0002-1395-4036[11]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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