Influence of ethylenediaminetetraacetic acid irrigation on the regenerative endodontic procedure in an immature rat molar model

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dc.contributor.authordos Reis-Prado, Alexandre Henrique
dc.contributor.authorOliveira, Sabrina de Castro
dc.contributor.authorGoto, Juliana [UNESP]
dc.contributor.authorSilva, Gerluza Aparecida Borges
dc.contributor.authorCintra, Luciano Tavares Angelo [UNESP]
dc.contributor.authorde Mesquita, Ricardo Alves
dc.contributor.authorSzawka, Raphael Escorsim
dc.contributor.authorRibeiro-Sobrinho, Antônio Paulino
dc.contributor.authorBenetti, Francine
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T15:12:12Z
dc.date.available2023-07-29T15:12:12Z
dc.date.issued2023-01-01
dc.description.abstractAim: To analyse the influence of ethylenediaminetetraacetic acid (EDTA) on the repair process in immature rat molars after a regenerative endodontic procedure (REP). Methodology: The lower first molars of 12 4-week-old Wistar rats underwent pulpectomy in the mesial root and were divided into the following groups: sodium hypochlorite (NaOCl; n = 6) – the mesial canals were irrigated with 2.5% NaOCl for 5 min, and NaOCl-EDTA (n = 6) – the canals were irrigated with 2.5% NaOCl, followed by 17% EDTA for 5 min each. After evoking bleeding using a size 10 K-file, the cavities were sealed. Three molars on the untreated side were randomly used as control (control-15 d; n = 3), and three molars from the other three rats untreated were used as immediate control (n = 3). After 15 days (NaOCl, NaOCl-EDTA and control-15 d groups) or immediately (control-immediate), the animals were euthanized, and the teeth were subjected to histologic evaluation of tissue regeneration and presence of collagen fibres. Mann–Whitney U-test was used (p <.05). Results: The experimental groups had newly formed cementum-like tissue and increased root length and thickness. Half of the specimens in NaOCl-EDTA group showed apical foramen closure, whilst the NaOCl group had partial apical closure. The experimental groups showed inflammatory infiltrate extending mainly to the medium third of the root canal. These parameters were similar between experimental groups (p >.05). Newly formed connective tissue in the pulp space was significantly higher in the NaOCl-EDTA group than in NaOCl group (p <.05). Regarding the collagen fibres, the NaOCl-EDTA group had more collagen fibres in the root tip, but there was no significant difference compared to NaOCl group, and both groups showed greater amount of immature fibres in this area; in the centre of the apical third of root canal, there was equivalence between mature and immature fibres from both groups (p >.05). Conclusions: Ethylenediaminetetraacetic acid irrigation improved newly formed intracanal connective tissue after REP in immature molars of rats; however, EDTA did not influence cementum-like tissue formation, apical closure, inflammatory infiltrate and maturation of collagen fibres.en
dc.description.affiliationRestorative Dentistry School of Dentistry Universidade Federal de Minas Gerais (UFMG)
dc.description.affiliationRestorative Dentistry School of Dentistry São Paulo State University (Unesp)
dc.description.affiliationMorphology Institute of Biological Sciences Universidade Federal de Minas Gerais (UFMG)
dc.description.affiliationOral Pathology and Surgery School of Dentistry Universidade Federal de Minas Gerais (UFMG)
dc.description.affiliationPhysiology and Biophysics Institute of Biological Sciences Universidade Federal de Minas Gerais (UFMG)
dc.description.affiliationUnespRestorative Dentistry School of Dentistry São Paulo State University (Unesp)
dc.format.extent69-79
dc.identifierhttp://dx.doi.org/10.1111/iej.13846
dc.identifier.citationInternational Endodontic Journal, v. 56, n. 1, p. 69-79, 2023.
dc.identifier.doi10.1111/iej.13846
dc.identifier.issn1365-2591
dc.identifier.issn0143-2885
dc.identifier.scopus2-s2.0-85140405772
dc.identifier.urihttp://hdl.handle.net/11449/249294
dc.language.isoeng
dc.relation.ispartofInternational Endodontic Journal
dc.sourceScopus
dc.subjectdental pulp
dc.subjectethylenediaminetetraacetic acid
dc.subjectguided tissue regeneration
dc.subjecthistologic outcomes
dc.subjectregenerative endodontics
dc.subjectroot canal irrigants
dc.titleInfluence of ethylenediaminetetraacetic acid irrigation on the regenerative endodontic procedure in an immature rat molar modelen
dc.typeArtigo
unesp.author.orcid0000-0002-5866-7137[1]
unesp.author.orcid0000-0003-2659-8322[2]
unesp.author.orcid0000-0002-6040-3560[3]
unesp.author.orcid0000-0003-2348-7846[5]
unesp.author.orcid0000-0003-3207-4007[6]
unesp.author.orcid0000-0002-2639-3469[7]
unesp.author.orcid0000-0002-3598-7592[8]
unesp.author.orcid0000-0002-5459-353X[9]

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