DAILY VARIATION IN PLASMA-CONCENTRATION OF FENCAMFAMINE AND STRIATAL DOPAMINE-RECEPTORS IN RATS

dc.contributor.authorPlaneta, Cleopatra da Silva [UNESP]
dc.contributor.authorDelucia, R.
dc.contributor.authorAizenstein, M. L.
dc.contributor.authorOliveira, G. H.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:20:09Z
dc.date.available2014-05-20T15:20:09Z
dc.date.issued1994-03-01
dc.description.abstractFencamfamine (FCF) is a psychostimulant drug classified as an indirect dopamine agonist. In the present study we evaluated the daily variation in plasma FCF concentration and in striatal dopamine receptors. Adult male Wistar rats (250-300 g) maintained on a 12-h light/12-h dark cycle (lights on at 07:00 h) were used. Rats received FCF (10.0 mg/kg, ip) at 09:00, 15:00, 21:00 or 03:00 h and blood samples were collected 30 (N = 6) or 60 (N = 6) min after the injections. Plasma FCF was measured by gas chromatography using an electron capture detector. Two-way ANOVA showed significant differences in FCF concentration when blood samples were collected 30 min after the injection, and the highest value was obtained following injection 21:00 h. Moreover, at 15:00, 21:00 and 03:00h, plasma FCF levels were significantly lower 60 min after injection when compared to the 30-min interval. Two other groups of rats (N = 6) were decapitated at 09:00 or 21:00 h and the striata were dissected for the binding assays. The Bmax for [H-3]-spiroperidol binding to striatal membranes was higher at 21:00 h, without changes in affinity constant (Kd). In conclusion, plasma FCF levels and dopamine receptors undergo daily variation,a phenomenon that should be considered to explain the circadian time-dependent effects of FCF.en
dc.description.affiliationUNIV SAO PAULO,INST CIENCIAS BIOMED,DEPT FARMACOL,BR-05508900 SAO PAULO,SP,BRAZIL
dc.format.extent737-741
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_issues&pid=0100-879X&lng=en&nrm=iso
dc.identifier.citationBrazilian Journal of Medical and Biological Research. São Paulo: Associação Bras Divulg Cientifica, v. 27, n. 3, p. 737-741, 1994.
dc.identifier.issn0100-879X
dc.identifier.lattes2514762545280942
dc.identifier.orcid0000-0002-1378-6327
dc.identifier.urihttp://hdl.handle.net/11449/31495
dc.identifier.wosWOS:A1994NB22600019
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica (ABRADIC)
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.relation.ispartofjcr1.492
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectFENCAMFAMINEpt
dc.subjectDOPAMINE RECEPTORSpt
dc.subjectCIRCADIAN RHYTHMpt
dc.subjectCHRONOPHARMACOLOGYpt
dc.subjectPLASMA FENCAMFAMINE LEVELSpt
dc.titleDAILY VARIATION IN PLASMA-CONCENTRATION OF FENCAMFAMINE AND STRIATAL DOPAMINE-RECEPTORS IN RATSen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/bjmbr/iaboutj.htm
dcterms.rightsHolderAssociação Bras Divulg Cientifica
unesp.author.lattes2514762545280942[1]
unesp.author.orcid0000-0002-1378-6327[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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