Cardiorespiratory effects of gap junction blockade in the locus coeruleus in unanesthetized adult rats

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dc.contributor.authorPatrone, Luis G. A. [UNESP]
dc.contributor.authorBicego, Kenia Cardoso [UNESP]
dc.contributor.authorHartzler, Lynn K.
dc.contributor.authorPutnam, Robert W. [UNESP]
dc.contributor.authorGargaglioni, Luciane H. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionWright State Univ
dc.date.accessioned2014-12-03T13:11:30Z
dc.date.available2014-12-03T13:11:30Z
dc.date.issued2014-01-01
dc.description.abstractThe locus coeruleus (LC) plays an important role in central chemoreception. In young rats (P9 or younger), 85% of LC neurons increase firing rate in response to hypercapnia vs. only about 45% of neurons from rats P10 or older. Carbenoxolone (CARB - gap junction blocker) does not affect the % of LC neurons responding in young rats but it decreases the % responding by half in older animals. We evaluated the participation of gap junctions in the CO2 ventilatory response in unanesthetized adult rats by bilaterally microinjecting CARB (300 mu M, 1 mM or 3 mM/100 nL), glycyrrhizic acid (GZA, CARB analog, 3 mM) or vehicle (aCSF - artificial cerebrospinal fluid) into the LC of Wistar rats. Bilateral gap junction blockade in LC neurons did not affect resting ventilation; however, the increase in ventilation produced by hypercapnia (7% CO2) was reduced by similar to 25% after CARB 1 mM or 3 mM injection (1939.7 +/- 104.8 mL kg(-1) min(-1) for the aCSF group and 1468.3 +/- 122.2 mL kg(-1) min(-1) for 1 mM CARB, P < 0.05; 1939.7 +/- 104.8 mL kg(-1) min(-1) for the aCSF group and 1540.9 +/- 68.4 mL kg(-1) min(-1) for the 3 mM CARB group, P < 0.05) due largely to a decrease in respiratory frequency. GZA injection or CARB injection outside the LC (peri-LC) had no effect on ventilation under any conditions. The results suggest that gap junctions in the LC modulate the hypercapnic ventilatory response of adult rats. (C) 2013 Elsevier B.V. All rights reserved.en
dc.description.affiliationSao Paulo State Univ, UNESP FCAV, Dept Anim Physiol & Morphol, Jaboticabal, SP, Brazil
dc.description.affiliationWright State Univ, Dept Biol Sci, Dayton, OH 45435 USA
dc.description.affiliationSao Paulo State Univ Jaboticabal, Dept Neurosci Cell Biol & Physiol, BR-14870000 Jaboticabal, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, UNESP FCAV, Dept Anim Physiol & Morphol, Jaboticabal, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ Jaboticabal, Dept Neurosci Cell Biol & Physiol, BR-14870000 Jaboticabal, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipINCT-Fisiologia Comparada
dc.description.sponsorshipIdFAPESP: 10/06210-9
dc.description.sponsorshipIdCNPq: 573921/2008-3 INCT-FisC
dc.format.extent86-95
dc.identifierhttp://dx.doi.org/10.1016/j.resp.2013.09.001
dc.identifier.citationRespiratory Physiology & Neurobiology. Amsterdam: Elsevier Science Bv, v. 190, n. 1, p. 86-95, 2014.
dc.identifier.doi10.1016/j.resp.2013.09.001
dc.identifier.issn1569-9048
dc.identifier.urihttp://hdl.handle.net/11449/113204
dc.identifier.wosWOS:000329004900013
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofRespiratory Physiology & Neurobiology
dc.relation.ispartofjcr1.792
dc.relation.ispartofsjr0,682
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCarbenoxoloneen
dc.subjectHypercapniaen
dc.subjectA6en
dc.subjectVentilationen
dc.subjectElectrical couplingen
dc.titleCardiorespiratory effects of gap junction blockade in the locus coeruleus in unanesthetized adult ratsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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