Effects of Laser Irradiation on Pulp Cells Exposed to Bleaching Agents

Página do item simplificado
dc.contributor.authorLima, Adriano F.
dc.contributor.authorBasso, Fernanda G.
dc.contributor.authorRibeiro, Ana P. D.
dc.contributor.authorBagnato, Vanderlei S.
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.authorMarchi, Giselle M.
dc.contributor.authorSouza Costa, Carlos A. de [UNESP]
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Nove de Julho (UNINOVE)
dc.contributor.institutionUniversidade de Brasília (UnB)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-12-03T13:10:45Z
dc.date.available2014-12-03T13:10:45Z
dc.date.issued2014-01-01
dc.description.abstractThe aim of this study was to evaluate the effect of low-level laser therapy (LLLT) on odontoblast-like cells exposed to a bleaching agent. Mouse dental papilla cell-23 cells were seeded in wells of 24-well plates. Eight groups were established according to the exposure to the bleaching agent and LLLT (0, 4, 10 and 15Jcm(-2)). Enamel-dentin disks were adapted to artificial pulp chambers, which were individually placed in wells containing Dulbecco's modified Eagle's medium (DMEM). A bleaching agent (35% hydrogen peroxide [BA35%HP]) was applied on enamel (15min) to obtain the extracts (DMEM+BA35%HP components diffused through enamel/dentin disks). The extracts were applied (1h) to the cells, and then subjected to LLLT. Cell viability (Methyl tetrazolium assay), alkaline phosphatase (ALP) activity, as well as gene expression of ALP, fibronectin (FN) and type I collagen, were evaluated. The bleaching procedures reduced the cell viability, ALP activity and gene expression of dentin proteins. Laser irradiation did not modulate the cell response; except for FN, as LLLT decreased the gene expression of this protein by the cells exposed to the BA35%HP. It can be concluded that BA35%HP decreased the activities of odontoblasts that were not recovered by the irradiation of the damaged cells with low-level laser parameters tested.en
dc.description.affiliationUniv Campinas UNICAMP, Piracicaba Dent Sch, Dept Restorat Dent, Piracicaba, Brazil
dc.description.affiliationNove de Julho Univ, Dept Restorat Dent, Sao Paulo, Brazil
dc.description.affiliationUniv Campinas UNICAMP, Piracicaba Dent Sch, Dept Pathol, Piracicaba, Brazil
dc.description.affiliationUniv Brasilia UnB, Dept Operat Dent, Brasilia, DF, Brazil
dc.description.affiliationUniv Sao Paulo, Phys Inst Sao Carlos, Sao Carlos, SP, Brazil
dc.description.affiliationUniv Estadual Paulista UNESP, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, Araraquara, Brazil
dc.description.affiliationUniv Estadual Paulista UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, Araraquara, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, Araraquara, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, Araraquara, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 09/08992-7-8
dc.description.sponsorshipIdFAPESP: 10/00645-3
dc.description.sponsorshipIdCNPq: 301291/2010-1
dc.format.extent201-206
dc.identifierhttp://dx.doi.org/10.1111/php.12155
dc.identifier.citationPhotochemistry And Photobiology. Hoboken: Wiley-blackwell, v. 90, n. 1, p. 201-206, 2014.
dc.identifier.doi10.1111/php.12155
dc.identifier.issn0031-8655
dc.identifier.lattes4517484241515548
dc.identifier.urihttp://hdl.handle.net/11449/112495
dc.identifier.wosWOS:000329368200026
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofPhotochemistry and Photobiology
dc.relation.ispartofjcr2.214
dc.relation.ispartofsjr0,591
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleEffects of Laser Irradiation on Pulp Cells Exposed to Bleaching Agentsen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.author.lattes4517484241515548[7]
unesp.author.orcid0000-0002-0945-1305[6]
unesp.author.orcid0000-0002-1507-2264[1]
unesp.author.orcid0000-0002-7455-6867[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

Arquivos

Coleções

Artigos - Clínica Infantil - FOAR
Artigos - Fisiologia e Patologia - FOAR