Attempts for increasing Astyanax altiparanae spawning rates and percentage of responsive oocytes

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dc.contributor.authorAriki, Daniel Guimarães Figueiredo [UNESP]
dc.contributor.authorRoza de Abreu, Mariana [UNESP]
dc.contributor.authorde Jesus Silva, Laíza Maria [UNESP]
dc.contributor.authorSato, Rafael Tomoda [UNESP]
dc.contributor.authorBatlouni, Sergio Ricardo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T13:58:16Z
dc.date.available2023-07-29T13:58:16Z
dc.date.issued2023-07-01
dc.description.abstractIn this study, we aimed to propose changes in the protocol of cultured Astyanax altiparanae hypophysation to increase the maximum ovulation rate of 60% registered previously. To that two consecutive experiments were conducted. In the first experiment, three carp pituitary homogenate (CPH) doses (3, 6, and 9 mg/kg) were administered in a single injection, while in the second experiment, the 6 mg/kg CPH dose was tested either in single or double injections. In the first experiment, a single injection of 3 mg/kg CPH did not induce final oocyte maturation or spawning, while a dose of 6 mg/kg CPH resulted in an increase in the plasma level of prostaglandin (PGF2α) at ovulation. The single higher dose of 9 mg/kg CPH did not improve reproductive performance and even though anticipated the resumption of meiosis it was detrimental to the spawning rate. In the second experiment, the dose of 6 mg/kg CPH fractionated into two injections led to a higher spawning rate, spawning volume per female body mass, frequency of post-ovulatory complexes, and PGF2α concentration at ovulation compared to the single injection. The most effective treatment remained the 6 mg/kg of CPH fractionated into two injections, but still providing very low proportion of ovulated females (∼40 %). Overall, this study indicates that the spawning protocols for this species need to be improved to induce ovulation in a larger number of females and be more potent in those females that respond positively.en
dc.description.affiliationCentro de Aquicultura da UNESP – CAUNESP Universidade Estadual Paulista – UNESP, Via de Acesso Prof. Paulo Donato Castellane, S/N, SP
dc.description.affiliationUnespCentro de Aquicultura da UNESP – CAUNESP Universidade Estadual Paulista – UNESP, Via de Acesso Prof. Paulo Donato Castellane, S/N, SP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPESP: #2016/25988–7
dc.description.sponsorshipIdCAPES: 001
dc.identifierhttp://dx.doi.org/10.1016/j.anireprosci.2023.107262
dc.identifier.citationAnimal Reproduction Science, v. 254.
dc.identifier.doi10.1016/j.anireprosci.2023.107262
dc.identifier.issn0378-4320
dc.identifier.scopus2-s2.0-85161286751
dc.identifier.urihttp://hdl.handle.net/11449/248951
dc.language.isoeng
dc.relation.ispartofAnimal Reproduction Science
dc.sourceScopus
dc.subjectHormonal induction
dc.subjectHyphophysation
dc.subjectLambari
dc.subjectPGF2α
dc.subjectProstaglandin
dc.titleAttempts for increasing Astyanax altiparanae spawning rates and percentage of responsive oocytesen
dc.typeArtigo

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