Mechanical and biological characterization of resin-modified glass-ionomer cement containing doxycycline hyclate

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dc.contributor.authorde Castilho, Aline R. F.
dc.contributor.authorDuque, Cristiane
dc.contributor.authorNegrini, Thais de C. [UNESP]
dc.contributor.authorSacono, Nancy T. [UNESP]
dc.contributor.authorde Paula, Andreia B.
dc.contributor.authorSacramento, Patricia A.
dc.contributor.authorde Souza Costal, Carlos A. [UNESP]
dc.contributor.authorSpolidorio, Denise M. P. [UNESP]
dc.contributor.authorPuppin-Rontani, Regina M.
dc.contributor.institutionUniversidade Federal Fluminense (UFF)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2013-09-30T18:31:37Z
dc.date.accessioned2014-05-20T13:45:02Z
dc.date.available2013-09-30T18:31:37Z
dc.date.available2014-05-20T13:45:02Z
dc.date.issued2012-02-01
dc.description.abstractObjectives: To characterize the mechanical and biological properties of a resin-modified glass ionomer cement (RMGIC) containing doxycycline'hyclate.Methods: The antibacterial effect of RMGIC containing 1.5, 3.0 and 4.5% doxycycline hyclate was assessed using two experiments - agar diffusion test for 24 h and biofilm assay for 24 h and 7 days - against some cariogenic bacteria. Briefly, base layers of BHI agar and 300 mu L of each inoculum were prepared in Petri dishes with 6 wells that were completely filled with materials. After 24 h incubation, zones of bacterial growth inhibition were measured using a digital caliper. Biofilm assays were conducted using RMGIC specimens immersed in 24-well plates containing the inoculum in BHI broth. After 24 h and 7 days, each specimen were removed, vortexed and the suspension diluted and inoculated in BHI plates for subsequent bacterial counting. Cytotoxicity tests used 50 specimens made in sterilized metal molds, including Vitrebond as positive control. Extracts from every specimen were applied on the MDPC-23 odontoblast-like cells for 24 h. The MIT assay and SEM evaluation determined cell metabolism and morphology, respectively. 80 cylindrical specimens were made from the previously cited groups, and were submitted to testing with a universal testing machine (Instron 4411) using a crosshead speed of 1.0 mm/min for compressive strength and 0.5 mm/mm for diametral tensile strength, respectively. Data from antibacterial and cytotoxic effects, and mechanical properties were submitted to appropriated statistical tests.Results: All tested groups showed growth inhibition of all tested strains (p < 0.05) in 24 h for both microbiological tests, but only 4.5% doxycycline have antibacterial effect after 7 days. None of doxycycline concentrations caused toxic effect to the MDPC-23 cells or presenting alterations to mechanical properties.Conclusion: The incorporation of up to 4.5% doxycycline hyclate into RMGIC inhibits important oral microorganisms, without modifying biological and mechanical characteristics of the dental material, suggesting a new alternative for the treatment of dental caries. (C) 2011 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUniv Fed Fluminense, Dept Dent, BR-28625650 Rio de Janeiro, Brazil
dc.description.affiliationUniv Campinas UNICAMP, Piracicaba Dent Sch, Dept Pediat Dent, BR-13414903 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista UNESP, Araraquara Sch Pharmaceut Sci, Dept Clin Anal, BR-14801902 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista UNESP, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, BR-14801903 São Paulo, Brazil
dc.description.affiliationUniv Campinas UNICAMP, Piracicaba Dent Sch, Dept Dent Mat, BR-13414903 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, BR-14801903 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Araraquara Sch Pharmaceut Sci, Dept Clin Anal, BR-14801902 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, BR-14801903 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, BR-14801903 São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 08/00359-0
dc.description.sponsorshipIdFAPESP: 08/02606-5
dc.format.extent131-138
dc.identifierhttp://dx.doi.org/10.1016/j.archoralbio.2011.08.009
dc.identifier.citationArchives of Oral Biology. Oxford: Pergamon-Elsevier B.V. Ltd, v. 57, n. 2, p. 131-138, 2012.
dc.identifier.doi10.1016/j.archoralbio.2011.08.009
dc.identifier.fileWOS000300801900003.pdf
dc.identifier.issn0003-9969
dc.identifier.urihttp://hdl.handle.net/11449/15804
dc.identifier.wosWOS:000300801900003
dc.language.isoeng
dc.publisherPergamon-Elsevier B.V. Ltd
dc.relation.ispartofArchives of Oral Biology
dc.relation.ispartofjcr2.050
dc.relation.ispartofsjr0,752
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectAntibioticen
dc.subjectGlass-ionomer cementen
dc.subjectAntibacterial activityen
dc.subjectCell cultureen
dc.subjectMechanical propertiesen
dc.titleMechanical and biological characterization of resin-modified glass-ionomer cement containing doxycycline hyclateen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderPergamon-Elsevier B.V. Ltd
unesp.author.lattes4517484241515548[7]
unesp.author.orcid0000-0003-2376-1024[8]
unesp.author.orcid0000-0002-7455-6867[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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Artigos - Análises Clínicas - FCFAR
Artigos - Clínica Infantil - FOAR
Artigos - Fisiologia e Patologia - FOAR