Comparative evaluation of enzyme-linked immunosorbent assay based on crude and purified antigen in the diagnosis of canine visceral leishmaniasis in symptomatic and oligosymptomatic dogs

Página do item simplificado
dc.contributor.authorCandido, Teresinha Cristina [UNESP]
dc.contributor.authorVenturoli Perri, Silvia Helena [UNESP]
dc.contributor.authorGerzoschkwitz, Tatiana de Oliveira
dc.contributor.authorLuvizotto, Maria Cecília Rui [UNESP]
dc.contributor.authorFelix de Lima, Valeria Marcal [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2013-09-30T18:25:25Z
dc.date.accessioned2014-05-20T13:42:56Z
dc.date.available2013-09-30T18:25:25Z
dc.date.available2014-05-20T13:42:56Z
dc.date.issued2008-11-07
dc.description.abstractThis study evaluated the performance of crude total antigen (CTA) and fucose-mannose ligand antigen (FML) in an enzyme-linked immunosorbent assay for diagnosis of canine visceral leishmaniasis (CVL). The assays used sera from known negative controls (n = 30), clinically symptomatic (n = 30) and oligosymptomatic (n = 30) parasitologically proven infection (by microscopy). Aspirates of popliteal lymph node from infected canines were colleted to score parasitism and compared with the ELISA results. The study indicated that FML used in ELISA provided high sensitivity for detecting oligosymptomatic dogs (90%) and CTA showed greater sensitivity than FML for symptomatic canines (90%). In oligosymptomatic dogs, specificity was 100% for CTA-ELISA, but in symptomatic dogs, FML specificity was higher (96.7%) than CTA-ELISA (93.3%). A significant correlation was observed between the degree of parasitism and the results obtained in CTA-ELISA. Since no available antigen offers 100% specificity and sensitivity for CVL diagnosis, the choice of antigen used must depend on the aim of the investigation. (C) 2008 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Estadual Paulista, Fac Odontol Aracatuba, Dept Clin Cirurg & Reprod Anim, BR-16050400 Aracatuba, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Odontol Aracatuba, Dept Apoio & Prod & Saúde Anim, BR-16050400 Aracatuba, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Odontol Aracatuba, Dept Clin Cirurg & Reprod Anim, BR-16050400 Aracatuba, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Odontol Aracatuba, Dept Apoio & Prod & Saúde Anim, BR-16050400 Aracatuba, SP, Brazil
dc.format.extent175-181
dc.identifierhttp://dx.doi.org/10.1016/j.vetpar.2008.08.010
dc.identifier.citationVeterinary Parasitology. Amsterdam: Elsevier B.V., v. 157, n. 3-4, p. 175-181, 2008.
dc.identifier.doi10.1016/j.vetpar.2008.08.010
dc.identifier.issn0304-4017
dc.identifier.lattes0810837598202634
dc.identifier.lattes4584674909952477
dc.identifier.urihttp://hdl.handle.net/11449/14924
dc.identifier.wosWOS:000260943600002
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofVeterinary Parasitology
dc.relation.ispartofjcr2.422
dc.relation.ispartofsjr1,275
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectDogen
dc.subjectVisceral leishmaniasisen
dc.subjectELISAen
dc.subjectFucose-mannose liganden
dc.titleComparative evaluation of enzyme-linked immunosorbent assay based on crude and purified antigen in the diagnosis of canine visceral leishmaniasis in symptomatic and oligosymptomatic dogsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes0810837598202634
unesp.author.lattes4584674909952477
unesp.author.orcid0000-0001-5083-5557[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatubapt

Arquivos

Licença do Pacote
Agora exibindo 1 - 2 de 2
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Clínica, Cirurgia e Reprodução Animal - FMVA
Artigos - Apoio, Produção e Saúde Animal - FMVA