Tissue Bioengineering with Fibrin Scaffolds and Deproteinized Bone Matrix Associated or Not with the Transoperative Laser Photobiomodulation Protocol

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dc.contributor.authorPomini, Karina Torres
dc.contributor.authorBuchaim, Daniela Vieira
dc.contributor.authorBighetti, Ana Carolina Cestari
dc.contributor.authorHamzé, Abdul Latif
dc.contributor.authorReis, Carlos Henrique Bertoni
dc.contributor.authorDuarte, Marco Antonio Húngaro
dc.contributor.authorAlcalde, Murilo Priori
dc.contributor.authorBarraviera, Benedito [UNESP]
dc.contributor.authorJúnior, Rui Seabra Ferreira [UNESP]
dc.contributor.authorde Souza, Alexandre Teixeira
dc.contributor.authorda Silva Santos, Paulo Sérgio
dc.contributor.authorPilon, João Paulo Galletti [UNESP]
dc.contributor.authorde Marchi, Miguel Ângelo
dc.contributor.authorNogueira, Dayane Maria Braz
dc.contributor.authorde Souza Bueno, Cleuber Rodrigo
dc.contributor.authorSoares, Wendel Cleber
dc.contributor.authorBuchaim, Rogerio Leone
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversity of Marilia (UNIMAR)
dc.contributor.institutionUniversity Center of Adamantina (UNIFAI)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUNINOVE University
dc.date.accessioned2023-07-29T13:35:47Z
dc.date.available2023-07-29T13:35:47Z
dc.date.issued2023-01-01
dc.description.abstractExtending the range of use of the heterologous fibrin biopolymer, this pre-clinical study showed a new proportionality of its components directed to the formation of scaffold with a lower density of the resulting mesh to facilitate the infiltration of bone cells, and combined with therapy by laser photobiomodulation, in order to accelerate the repair process and decrease the morphofunctional recovery time. Thus, a transoperative protocol of laser photobiomodulation (L) was evaluated in critical bone defects filled with deproteinized bovine bone particles (P) associated with heterologous fibrin biopolymer (HF). The groups were: BCL (blood clot + laser); HF; HFL; PHF (P+HF); PHFL (P+HF+L). Microtomographically, bone volume (BV) at 14 days, was higher in the PHF and PHFL groups (10.45 ± 3.31 mm3 and 9.94 ± 1.51 mm3), significantly increasing in the BCL, HFL and PHFL groups. Histologically, in all experimental groups, the defects were not reestablished either in the external cortical bone or in the epidural, occurring only in partial bone repair. At 42 days, the bone area (BA) increased in all groups, being significantly higher in the laser-treated groups. The quantification of bone collagen fibers showed that the percentage of collagen fibers in the bone tissue was similar between the groups for each experimental period, but significantly higher at 42 days (35.71 ± 6.89%) compared to 14 days (18.94 ± 6.86%). It can be concluded that the results of the present study denote potential effects of laser radiation capable of inducing functional bone regeneration, through the synergistic combination of biomaterials and the new ratio of heterologous fibrin biopolymer components (1:1:1) was able to make the resulting fibrin mesh less dense and susceptible to cellular permeability. Thus, the best fibrinogen concentration should be evaluated to find the ideal heterologous fibrin scaffold.en
dc.description.affiliationDepartment of Biological Sciences Bauru School of Dentistry (FOB/USP) University of São Paulo
dc.description.affiliationPostgraduate Program in Structural and Functional Interactions in Rehabilitation Postgraduate Department University of Marilia (UNIMAR)
dc.description.affiliationTeaching and Research Coordination of the Medical School University Center of Adamantina (UNIFAI)
dc.description.affiliationMedical School University of Marilia (UNIMAR)
dc.description.affiliationUNIMAR Beneficent Hospital (HBU) University of Marilia (UNIMAR)
dc.description.affiliationDepartment of Dentistry Endodontics and Dental Materials Bauru School of Dentistry University of São Paulo (FOB/USP)
dc.description.affiliationCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (University Estadual Paulista UNESP)
dc.description.affiliationGraduate Programs in Tropical Diseases and Clinical Research Botucatu Medical School (FMB) São Paulo State University (UNESP–University Estadual Paulista)
dc.description.affiliationUniversity Center of Adamantina (UNIFAI)
dc.description.affiliationDepartment of Surgery Stomatology Pathology and Radiology Bauru School of Dentistry University of São Paulo
dc.description.affiliationPostgraduate Program in Speech Therapy São Paulo State University (UNESP—University Estadual Paulista)
dc.description.affiliationCoordination of the Medical School University Center of Adamantina (UNIFAI)
dc.description.affiliationDepartment of Prosthodontics and Periodontics Bauru School of Dentistry (FOB/USP) University of São Paulo
dc.description.affiliationAnatomy and Collective Health Faculty of Medicine and Dentistry University Center of Adamantina (UNIFAI)
dc.description.affiliationAnatomy Department Faculty of Medicine UNINOVE University
dc.description.affiliationGraduate Program in Anatomy of Domestic and Wild Animals Faculty of Veterinary Medicine and Animal Science University of São Paulo (FMVZ/USP)
dc.description.affiliationUnespCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (University Estadual Paulista UNESP)
dc.description.affiliationUnespGraduate Programs in Tropical Diseases and Clinical Research Botucatu Medical School (FMB) São Paulo State University (UNESP–University Estadual Paulista)
dc.description.affiliationUnespPostgraduate Program in Speech Therapy São Paulo State University (UNESP—University Estadual Paulista)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: 303224/2018-5
dc.description.sponsorshipIdCNPq: 306339/2020-0
dc.identifierhttp://dx.doi.org/10.3390/molecules28010407
dc.identifier.citationMolecules, v. 28, n. 1, 2023.
dc.identifier.doi10.3390/molecules28010407
dc.identifier.issn1420-3049
dc.identifier.scopus2-s2.0-85145733889
dc.identifier.urihttp://hdl.handle.net/11449/248148
dc.language.isoeng
dc.relation.ispartofMolecules
dc.sourceScopus
dc.subjectbiocompatible materials
dc.subjectbiopolymers
dc.subjectbone regeneration
dc.subjectbone substitutes
dc.subjectfibrin sealant
dc.subjectlow-level laser therapy
dc.subjectphotobiomodulation
dc.subjectxenografts
dc.titleTissue Bioengineering with Fibrin Scaffolds and Deproteinized Bone Matrix Associated or Not with the Transoperative Laser Photobiomodulation Protocolen
dc.typeArtigo
unesp.author.orcid0000-0003-2858-4717[1]
unesp.author.orcid0000-0002-9914-1262[2]
unesp.author.orcid0000-0003-3051-737X[6]
unesp.author.orcid0000-0001-6952-0512[9]
unesp.author.orcid0000-0002-0674-3759[11]
unesp.author.orcid0000-0002-8963-8793[15]
unesp.author.orcid0000-0002-5881-2218[17]

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Botucatu - CEVAP - Centro de Estudos de Venenos e Animais Peçonhentos
Artigos - Doenças Tropicais e Diagnósticos por Imagem - FMB
Artigos - Fonoaudiologia - FFC