Babesia bovis infection in cattle in the southwestern Brazilian Amazon

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dc.contributor.authorBrito, Luciana G.
dc.contributor.authorRocha, Rodrigo B.
dc.contributor.authorBarbieri, Fábio da S.
dc.contributor.authorRibeiro, Elisana S.
dc.contributor.authorVendrami, Fabiano B.
dc.contributor.authorSouza, Gislaine C.R.
dc.contributor.authorGiglioti, Rodrigo [UNESP]
dc.contributor.authorRegitano, Luciana C.A.
dc.contributor.authorFalcoski, Thaís O.R.S. [UNESP]
dc.contributor.authorTizioto, Polyana C.
dc.contributor.authorOliveira, Márcia C.S.
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionAgência de Defesa Sanitária Agrosilvopastoril do Estado de Rondônia
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.date.accessioned2014-05-27T11:28:18Z
dc.date.available2014-05-27T11:28:18Z
dc.date.issued2013-02-01
dc.description.abstractThe present study provides the first epidemiological data on infection with Babesia bovis in cattle raised in the southwestern Brazilian Amazon. Blood clot samples were filtered through nylon cloth before being submitted to DNA extraction. PCR and nested-PCR were applied to assess the frequency of infection with B. bovis in calves with ages from 4 to 12 months bred in 4 microregions each in the states of Rondônia and Acre. After the DNA was extracted from the samples, the infection in cattle was investigated by amplification of the rap1 gene from B. bovis. The DNA amplification results revealed a frequency of infection with B. bovis of 95.1% (272/286) in the samples from Rondônia and 96.1% (195/203) in those from Acre. The high frequency of B. bovis infection in the animals with ages from 4 to 12 months indicates a situation of enzootic stability in the regions studied. The infection rates are comparable to those detected by immunodiagnostic techniques in other endemic regions of Brazil. © 2012 Elsevier GmbH.en
dc.description.affiliationEmbrapa Rondônia, Rodovia BR 364, km 5, 5, Caixa Postal 127, 76815-800, Porto Velho, RO
dc.description.affiliationAgência de Defesa Sanitária Agrosilvopastoril do Estado de Rondônia, Porto Velho, RO
dc.description.affiliationUniversidade Estadual Júlio de Mesquita Filho, Jaboticabal, SP
dc.description.affiliationEmbrapa Pecuária Sudeste, São Carlos, SP
dc.description.affiliationUniversidade Estadual Júlio de Mesquita Filho, Araraquara, SP
dc.description.affiliationUniversidade Federal de São Carlos, São Carlos, SP
dc.description.affiliationUnespUniversidade Estadual Júlio de Mesquita Filho, Jaboticabal, SP
dc.description.affiliationUnespUniversidade Estadual Júlio de Mesquita Filho, Araraquara, SP
dc.format.extent78-82
dc.identifierhttp://dx.doi.org/10.1016/j.ttbdis.2012.08.001
dc.identifier.citationTicks and Tick-borne Diseases, v. 4, n. 1-2, p. 78-82, 2013.
dc.identifier.doi10.1016/j.ttbdis.2012.08.001
dc.identifier.issn1877-959X
dc.identifier.issn1877-9603
dc.identifier.scopus2-s2.0-84872612113
dc.identifier.urihttp://hdl.handle.net/11449/74483
dc.identifier.wosWOS:000316519000011
dc.language.isoeng
dc.relation.ispartofTicks and Tick-borne Diseases
dc.relation.ispartofjcr2.612
dc.relation.ispartofsjr1,421
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAmazonia
dc.subjectBabesia bovis
dc.subjectBrazil
dc.subjectCattle
dc.subjectEpidemiology
dc.subjectRap1 protein
dc.subjectblood clot
dc.subjectblood sampling
dc.subjectcalf (bovine)
dc.subjectcattle
dc.subjectcontrolled study
dc.subjectDNA extraction
dc.subjectDNA isolation
dc.subjectgene
dc.subjectgene amplification
dc.subjectinfection rate
dc.subjectnested polymerase chain reaction
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectpiroplasmosis
dc.subjectpolymerase chain reaction
dc.subjectpriority journal
dc.subjectrap1 gene
dc.subjectsensitivity and specificity
dc.subjectserodiagnosis
dc.subjectAnimals
dc.subjectBabesiosis
dc.subjectCattle Diseases
dc.subjectDNA, Protozoan
dc.subjectPolymerase Chain Reaction
dc.subjectSensitivity and Specificity
dc.subjectAnimalia
dc.subjectBos
dc.titleBabesia bovis infection in cattle in the southwestern Brazilian Amazonen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy

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