Fungal Screening for Potential PET Depolymerization

Página do item simplificado
dc.contributor.authorMalafatti-Picca, Lusiane [UNESP]
dc.contributor.authorBucioli, Elaine Cristina [UNESP]
dc.contributor.authorde Barros Chaves, Michel Ricardo
dc.contributor.authorde Castro, Aline Machado
dc.contributor.authorValoni, Érika
dc.contributor.authorde Oliveira, Valéria Maia
dc.contributor.authorMarsaioli, Anita Jocelyne
dc.contributor.authorGovone, José Silvio [UNESP]
dc.contributor.authorde Franceschi de Angelis, Dejanira [UNESP]
dc.contributor.authorBrienzo, Michel [UNESP]
dc.contributor.authorAttili-Angelis, Derlene [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionFederal University of Maranhão (UFMA)
dc.contributor.institutionPETROBRAS
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2023-07-29T13:51:05Z
dc.date.available2023-07-29T13:51:05Z
dc.date.issued2023-03-01
dc.description.abstractApproximately 400 billion PET bottles are produced annually in the world, of which from 8 to 9 million tons are discarded in oceans. This requires developing strategies to urgently recycle them. PET recycling can be carried out using the microbial hydrolysis of polymers when monomers and oligomers are released. Exploring the metabolic activity of fungi is an environmentally friendly way to treat harmful polymeric waste and obtain the production of monomers. The present study addressed: (i) the investigation of potential of strains with the potential for the depolymerization of PET bottles from different manufacturers (crystallinity of 35.5 and 10.4%); (ii) the search for a culture medium that favors the depolymerization process; and (iii) gaining more knowledge on fungal enzymes that can be applied to PET recycling. Four strains (from 100 fungal strains) were found as promising for conversion into terephthalic acid from PET nanoparticles (npPET): Curvularia trifolii CBMAI 2111, Trichoderma sp. CBMAI 2071, Trichoderma atroviride CBMAI 2073, and Cladosporium cladosporioides CBMAI 2075. The fermentation assays in the presence of PET led to the release of terephthalic acid in concentrations above 12 ppm. Biodegradation was also confirmed using mass variation analyses (reducing mass), scanning electron microscopy (SEM) that showed evidence of material roughness, FTIR analysis that showed band modification, enzymatic activities detected for lipase, and esterase and cutinase, confirmed by monomers/oligomers quantification using high performance liquid chromatography (HPLC-UV). Based on the microbial strains PET depolymerization, the results are promising for the exploration of the selected microbial strain.en
dc.description.affiliationEnvironmental Studies Center (CEA) São Paulo State University (UNESP), Av. 24-A, 1515, Bela Vista, SP
dc.description.affiliationCoordination of Natural Sciences Federal University of Maranhão (UFMA), Av. João Alberto, 700, MA
dc.description.affiliationDepartment of Biotechnology RD Center PETROBRAS, Av. Horácio Macedo, 950, Ilha do Fundão, RJ
dc.description.affiliationDivision of Microbial Resources CPQBA State University of Campinas (Unicamp), Rua Alexandre Cazellato, 999, SP
dc.description.affiliationInstitute of Chemistry State University of Campinas (Unicamp), P.O. Box 6154, SP
dc.description.affiliationDepartment of Biochemistry and Microbiology São Paulo State University (UNESP), Av. 24-A, 1515, Bela Vista, SP
dc.description.affiliationInstitute For Research in Bioenergy (IPBEN) São Paulo State University (UNESP), R. 10, 2527, Santana, SP
dc.description.affiliationUnespEnvironmental Studies Center (CEA) São Paulo State University (UNESP), Av. 24-A, 1515, Bela Vista, SP
dc.description.affiliationUnespDepartment of Biochemistry and Microbiology São Paulo State University (UNESP), Av. 24-A, 1515, Bela Vista, SP
dc.description.affiliationUnespInstitute For Research in Bioenergy (IPBEN) São Paulo State University (UNESP), R. 10, 2527, Santana, SP
dc.description.sponsorshipPetrobras
dc.description.sponsorshipIdPetrobras: 2012/00327-7
dc.identifierhttp://dx.doi.org/10.3390/polym15061581
dc.identifier.citationPolymers, v. 15, n. 6, 2023.
dc.identifier.doi10.3390/polym15061581
dc.identifier.issn2073-4360
dc.identifier.scopus2-s2.0-85152629953
dc.identifier.urihttp://hdl.handle.net/11449/248696
dc.language.isoeng
dc.relation.ispartofPolymers
dc.sourceScopus
dc.subjectbiodegradation
dc.subjectenzymatic catalysis
dc.subjectpolymers
dc.subjectterephthalic acid
dc.titleFungal Screening for Potential PET Depolymerizationen
dc.typeArtigo
unesp.author.orcid0000-0002-1355-8455[4]
unesp.author.orcid0000-0002-3096-8843[10]

Arquivos

Coleções

Artigos - Bioquímica e Microbiologia - IBRC