Assessment of the regenerative potential of macro-porous chitosan-calcium simvastatin scaffolds on bone cells

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dc.contributor.authorDe Oliveira Gallinari, Marjorie
dc.contributor.authorBordini, Ester Alves Ferreira
dc.contributor.authorDe Toledo Stuani, Vitor
dc.contributor.authorCassiano, Fernanda Balestrero
dc.contributor.authorDe Melo, Camila Correa Da Silva Braga
dc.contributor.authorDe Almeida, Juliano Milanezi [UNESP]
dc.contributor.authorCintra, Luciano Tavares Ângelo [UNESP]
dc.contributor.authorDe Souza Costa, Carlos Alberto [UNESP]
dc.contributor.authorSoares, Diana Gabriela
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T13:42:11Z
dc.date.available2023-07-29T13:42:11Z
dc.date.issued2023-01-01
dc.description.abstractThis study evaluated the bioactive potential of a macro-porous chitosan scaffold incorporated with calcium hydroxide (CH-Ca) and functionalized with bioactive doses of simvastatin (SV) for bone tissue regeneration. Initially, the bioactive dose of SV in osteoblastic cells (SAOS-2) was determined. For the direct contact experiment, SAOS-2 cells were plated on scaffolds to assess cell viability and osteogenic differentiation. The second assay was performed at a distance using extracts from scaffolds incubated in culture medium to assess the effect of conditioned medium on viability and osteogenic differentiation. The initial screening showed that 1 μM SV presented the best biostimulating effects, and this dose was selected for incorporation into the CH-Ca and pure chitosan (CH) scaffolds. The cells remained viable throughout the direct contact experiment, with the greatest cell density in the CH-Ca and CH-Ca-SV scaffolds because of their higher porosity. The CH-Ca-SV scaffold showed the most intense bio-stimulating effect in assays in the presence and absence of osteogenic medium, leading to an increased deposition of mineralized matrix. There was an increase in the viability of cells exposed to the extracts for CH-Ca, CH-SV, and CH-Ca-SV during the one-day period. There was an increase in ALP activity in the CH-Ca and CH-Ca-SV; however, the CH-Ca-SV scaffold resulted in an intense increase in the deposition of mineralized nodules, approximately 56.4% at 7 days and 117% at 14 days, compared with CH (control). In conclusion, functionalization of the CH-Ca scaffold with SV promoted an increase in bioactivity, presenting a promising option for bone tissue regenerationen
dc.description.affiliationUniversidade de São Paulo USP Bauru School of Dentistry Department of Operative Dentistry Endodontics and Dental Materials, SP
dc.description.affiliationUniversidade Estadual Paulista Unesp Araçatuba School of Dentistry Department of Diagnosis and Surgery, SP
dc.description.affiliationUniversidade Estadual Paulista Unesp Araçatuba School of Dentistry Department of Preventive and Operative Dentistry, SP
dc.description.affiliationUniversidade Estadual Paulista Unesp Araraquara School of Dentistry Department of Physiology and Pathology, SP
dc.description.affiliationUnespUniversidade Estadual Paulista Unesp Araçatuba School of Dentistry Department of Diagnosis and Surgery, SP
dc.description.affiliationUnespUniversidade Estadual Paulista Unesp Araçatuba School of Dentistry Department of Preventive and Operative Dentistry, SP
dc.description.affiliationUnespUniversidade Estadual Paulista Unesp Araraquara School of Dentistry Department of Physiology and Pathology, SP
dc.identifierhttp://dx.doi.org/10.1590/1807-3107BOR-2023.VOL37.0018
dc.identifier.citationBrazilian Oral Research, v. 37.
dc.identifier.doi10.1590/1807-3107BOR-2023.VOL37.0018
dc.identifier.issn1807-3107
dc.identifier.issn1806-8324
dc.identifier.scopus2-s2.0-85148250689
dc.identifier.urihttp://hdl.handle.net/11449/248370
dc.language.isoeng
dc.relation.ispartofBrazilian Oral Research
dc.sourceScopus
dc.subjectChitosan
dc.subjectSimvastatin
dc.subjectTissue Engineering
dc.titleAssessment of the regenerative potential of macro-porous chitosan-calcium simvastatin scaffolds on bone cellsen
dc.typeArtigo

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