Anthelmintic effect of plant extracts containing condensed and hydrolyzable tannins on Caenorhabditis elegans, and their antioxidant capacity

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dc.contributor.authorKatiki, Luciana M.
dc.contributor.authorFerreira, Jorge F.S.
dc.contributor.authorGonzalez, Javier M.
dc.contributor.authorZajac, Anne M.
dc.contributor.authorLindsay, David S.
dc.contributor.authorChagas, Ana Carolina S.
dc.contributor.authorAmarante, Alessandro F.T. [UNESP]
dc.contributor.institutionAgência Paulista de Tecnologia dos Agronegócios (APTA)
dc.contributor.institutionAppalachian Farming Systems Research Center (USDA-ARS)
dc.contributor.institutionVirginia Tech
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionNational Soil Erosion Research Lab
dc.date.accessioned2014-05-27T11:28:27Z
dc.date.available2014-05-27T11:28:27Z
dc.date.issued2013-02-18
dc.description.abstractAlthough tannin-rich forages are known to increase protein uptake and to reduce gastrointestinal nematode infections in grazing ruminants, most published research involves forages with condensed tannins (CT), while published literature lacks information on the anthelmintic capacity, nutritional benefits, and antioxidant capacity of alternative forages containing hydrolyzable tannins (HT). We evaluated the anthelmintic activity and the antioxidant capacity of plant extracts containing either mostly CT, mostly HT, or both CT and HT. Extracts were prepared with 70% acetone, lyophilized, redissolved to doses ranging from 1.0mg/mL to 25mg/mL, and tested against adult Caenorhabditis elegans as a test model. The extract concentrations that killed 50% (LC50) or 90% (LC90) of the nematodes in 24h were determined and compared to the veterinary anthelmintic levamisole (8mg/mL). Extracts were quantified for CT by the acid butanol assay, for HT (based on gallic acid and ellagic acid) by high-performance liquid chromatography (HPLC) and total phenolics, and for their antioxidant activity by the oxygen radical absorbance capacity (ORAC) assay. Extracts with mostly CT were Lespedeza cuneata, Salix X sepulcralis, and Robinia pseudoacacia. Extracts rich in HT were Acer rubrum, Rosa multiflora, and Quercus alba, while Rhus typhina had both HT and CT. The extracts with the lowest LC50 and LC90 concentrations, respectively, in the C. elegans assay were Q. alba (0.75 and 1.06mg/mL), R. typhina collected in 2007 (0.65 and 2.74mg/mL), A. rubrum (1.03 and 5.54mg/mL), and R. multiflora (2.14 and 8.70mg/mL). At the doses of 20 and 25mg/mL, HT-rich, or both CT- and HT-rich, extracts were significantly more lethal to adult C. elegans than extracts containing only CT. All extracts were high in antioxidant capacity, with ORAC values ranging from 1800μmoles to 4651μmoles of trolox equivalents/g, but ORAC did not correlate with anthelmintic activity. The total phenolics test had a positive and highly significant (r=0.826, p≤0.01) correlation with total hydrolyzable tannins. Plants used in this research are naturalized to the Appalachian edaphoclimatic conditions, but occur in temperate climate areas worldwide. They represent a rich, renewable, and unexplored source of tannins and antioxidants for grazing ruminants, whereas conventional CT-rich forages, such as L. cuneata, may be hard to establish and adapt to areas with temperate climate. Due to their high in vitro anthelmintic activity, antioxidant capacity, and their adaptability to non-arable lands, Q. alba, R. typhina, A. rubrum, and R. multiflora have a high potential to improve the health of grazing animals and must have their anthelmintic effects confirmed in vivo in both sheep and goats. © 2012.en
dc.description.affiliationInstituto de Zootecnia (SAA-APTA), Rua Heitor Penteado 56, Nova Odessa, SP 13460-000
dc.description.affiliationAppalachian Farming Systems Research Center (USDA-ARS), 1224 Airport Rd., Beaver, WV 25813
dc.description.affiliationVirginia-Maryland Regional College of Veterinary Medicine Virginia Tech, Blacksburg, VA 24061-0442
dc.description.affiliationEmbrapa Pecuária Sudeste, Rod. Washington Luiz km 234, São Carlos, SP
dc.description.affiliationUNESP Universidade Estadual Paulista Departamento de Parasitologia, Botucatu, SP
dc.description.affiliationUSDA ARS National Soil Erosion Research Lab, 275 S. Russell St., West Lafayette, IN 47907
dc.description.affiliationUnespUNESP Universidade Estadual Paulista Departamento de Parasitologia, Botucatu, SP
dc.format.extent218-227
dc.identifierhttp://dx.doi.org/10.1016/j.vetpar.2012.09.030
dc.identifier.citationVeterinary Parasitology, v. 192, n. 1-3, p. 218-227, 2013.
dc.identifier.doi10.1016/j.vetpar.2012.09.030
dc.identifier.issn0304-4017
dc.identifier.issn1873-2550
dc.identifier.scopus2-s2.0-84871702948
dc.identifier.urihttp://hdl.handle.net/11449/74603
dc.identifier.wosWOS:000314078900026
dc.language.isoeng
dc.relation.ispartofVeterinary Parasitology
dc.relation.ispartofjcr2.422
dc.relation.ispartofsjr1,275
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAnthelmintic plants
dc.subjectAntioxidant capacity
dc.subjectC. elegans
dc.subjectCondensed tannins
dc.subjectHydrolyzable tannins
dc.subjectNematodes
dc.subjectORAC
dc.subjectSmall ruminants
dc.subjectTotal phenolics
dc.subjectTree tannins
dc.subjectAcer rubrum extract
dc.subjectacetone
dc.subjectellagic acid
dc.subjectgallic acid
dc.subjectLespedeza cuneata extract
dc.subjectlevamisole
dc.subjectphenol derivative
dc.subjectplant extract
dc.subjectQuercas alba extract
dc.subjectRhus typhina extract
dc.subjectRobinia pseudoacacia extract
dc.subjectRosa multiflora extract
dc.subjectSalix X sepulcralis extract
dc.subjecttannin derivative
dc.subjecttrolox C
dc.subjectunclassified drug
dc.subjectAcer rubrum
dc.subjectanthelmintic activity
dc.subjectantioxidant activity
dc.subjectCaenorhabditis elegans
dc.subjectconcentration (parameters)
dc.subjectcontrolled study
dc.subjecthigh performance liquid chromatography
dc.subjectin vitro study
dc.subjectLC 50
dc.subjectLC 90
dc.subjectlespedeza cuneata
dc.subjectmedicinal plant
dc.subjectnonhuman
dc.subjectplant leaf
dc.subjectQuercas alba
dc.subjectRhus typhina
dc.subjectRobinia pseudoacacia
dc.subjectRosa multiflora
dc.subjectSalix X sepulcralis
dc.subjectAnimalia
dc.subjectCapra hircus
dc.subjectLespedeza cuneata
dc.subjectNematoda
dc.subjectOvis aries
dc.subjectQuercus alba
dc.subjectSalix x sepulcralis
dc.titleAnthelmintic effect of plant extracts containing condensed and hydrolyzable tannins on Caenorhabditis elegans, and their antioxidant capacityen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.lattes2677231663329706[7]
unesp.author.orcid0000-0003-4550-6761[2]
unesp.author.orcid0000-0002-0592-8321[5]
unesp.author.orcid0000-0003-3939-0088[6]
unesp.author.orcid0000-0003-3995-5501[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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