Modulation of the activity and selectivity of the immobilized lipases by surfactants and solvents

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dc.contributor.authorQuilles Júnior, José Carlos [UNESP]
dc.contributor.authorBrito, R. R. [UNESP]
dc.contributor.authorBorges, J. P. [UNESP]
dc.contributor.authorAragon, C. C. [UNESP]
dc.contributor.authorFernandez-Lorente, G.
dc.contributor.authorBocchini-Martins, D. A. [UNESP]
dc.contributor.authorGomes, Eleni [UNESP]
dc.contributor.authorSilva, Roberto da [UNESP]
dc.contributor.authorBoscolo, Mauricio [UNESP]
dc.contributor.authorGuisan, J. M.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-04-27T11:56:06Z
dc.date.available2015-04-27T11:56:06Z
dc.date.issued2015
dc.description.abstractMost of lipases are in equilibrium between a majority inactive closed form and a minority active open form in aqueous media. Perhaps, a certain stabilization of these open forms of lipases could be achieved in the presence of cosolvents or surfactants in the reaction medium. Three commercial lipases were studied (from Thermomyces lanuginosa (TLL), Candida Antarctica fraction B (CALB) and Lecitase (LEC)). Different derivatives were tested: TLL and LEC were adsorbed on an anionic exchanger and their activity strongly depends on the equilibrium between their open and closed form and CALB was adsorbed on a hydrophobic support when the open form was already stabilized by the support. Derivatives ionically adsorbed were hyperactivated by surfactans as well as by cosolvents: the activity of LEC increased 12 times in the presence of 15–20% of ethanol. CALB adsorbed on hydrophobic supports was hardly hyperactivated and even it was inhibited. The modification of the rate of covalent modification of the catalytic Ser seems to confirm that the observed hyperactivations were due to a stabilization of the open form of the adsorbed lipases (TLL and LEC). The hydrolysis of sardine oil was also studied in the presence or absence of surfactants and cosolvents. An interesting improvement in the ability of derivatives to discriminate the release of eicosipentaenic acid (EPA) and docosahexaenicacid (DHA) was found.en
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Biologia, Instituto de Biociências Letras e Ciências Exatas de São José do Rio Preto, Sao Jose do Rio Preto, RUA CRISTOVAO COLOMBO, 2265, JARDIM NAZARETH, CEP 15054-000, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Biologia, Instituto de Biociências Letras e Ciências Exatas de São José do Rio Preto, Sao Jose do Rio Preto, RUA CRISTOVAO COLOMBO, 2265, JARDIM NAZARETH, CEP 15054-000, SP, Brasil
dc.description.affiliationUnespIQ/UNESP, Departament of Biochemistry and Chemical Technology—Rua Prof. Francisco Degni, 55—CEP, 14800-060 Araraquara, SP, Brazil
dc.description.affiliationUnespDepartament of Biotechnology and Food Microbiology, Research Institute for Food Science, CIAL-CSIC, Campus UAM, 28049 Madrid, Spain
dc.description.affiliationUnespDepartament of Catalysis, CSIC (Consejo Superior de Investigaciones Científicas), Campus UAM, 28049 Madrid, Spain
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2013/00530-0
dc.format.extent274-280
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S1369703X14002939
dc.identifier.citationBiochemical Engineering Journal, v. 93, p. 274-280, 2015.
dc.identifier.doi10.1016/j.bej.2014.10.009
dc.identifier.issn1369-703X
dc.identifier.lattes7091241742851920
dc.identifier.lattes9424175688206545
dc.identifier.lattes8880074921989984
dc.identifier.urihttp://hdl.handle.net/11449/122852
dc.language.isoeng
dc.relation.ispartofBiochemical Engineering Journal
dc.relation.ispartofjcr3.226
dc.rights.accessRightsAcesso aberto
dc.sourceCurrículo Lattes
dc.subjectLipasesen
dc.subjectImmobilizationen
dc.subjectStabilityen
dc.subjectSelectivityen
dc.subjectSurfactantsen
dc.subjectHydrolysis of fish oilen
dc.titleModulation of the activity and selectivity of the immobilized lipases by surfactants and solventsen
dc.typeArtigo
unesp.author.lattes7091241742851920
unesp.author.lattes9424175688206545
unesp.author.lattes8880074921989984
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt
unesp.departmentBiologiapt

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