Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances

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dc.contributor.authorVidal, D.
dc.contributor.authorFortunato, G.
dc.contributor.authorKlein, W. [UNESP]
dc.contributor.authorCortizo, L.
dc.contributor.authorVasconcelos, J.
dc.contributor.authorRibeiro-dos-Santos, R.
dc.contributor.authorSoares, M.
dc.contributor.authorMacambira, S.
dc.contributor.institutionFundação Oswaldo Cruz
dc.contributor.institutionHosp Sao Rafael
dc.contributor.institutionUniv Estadual Feira de Santana
dc.contributor.institutionUniversidade Federal da Bahia (UFBA)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.date.accessioned2014-05-20T15:32:32Z
dc.date.available2014-05-20T15:32:32Z
dc.date.issued2012-05-01
dc.description.abstractSeveral experimental studies of pulmonary emphysema using animal models have been described in the literature. However, only a few of these studies have focused on the assessment of ergometric function as a non-invasive technique to validate the methodology used for induction of experimental emphysema. Additionally, functional assessments of emphysema are rarely correlated with morphological pulmonary abnormalities caused by induced emphysema. The present study aimed to evaluate the effects of elastase administered by tracheal puncture on pulmonary parenchyma and their corresponding functional impairment. This was evaluated by measuring exercise capacity in C57Bl/6 mice in order to establish a reproducible and safe methodology of inducing experimental emphysema. Thirty six mice underwent ergometric tests before and 28 days after elastase administration. Pancreatic porcine elastase solution was administered by tracheal puncture, which resulted in a significantly decreased exercise capacity, shown by a shorter distance run (-30.5%) and a lower mean velocity (-15%), as well as in failure to increase the elimination of carbon dioxide. The mean linear intercept increased significantly by 50% in tracheal elastase administration. In conclusion, application of elastase by tracheal function in C57Bl/6 induces emphysema, as validated by morphometric analyses, and resulted in a significantly lower exercise capacity, while resulting in a low mortality rate. (C) 2011 Sociedade Portuguesa de Pneumologia. Published by Elsevier Espana, S.L. All rights reserved.en
dc.description.affiliationFundação Oswaldo Cruz, Lab Engn Tecidual & Imunofarmacol, Ctr Pesquisas Goncalo Moniz, Salvador, BA, Brazil
dc.description.affiliationHosp Sao Rafael, Ctr Biotecnol & Terapia Celular, Salvador, BA, Brazil
dc.description.affiliationUniv Estadual Feira de Santana, Programa Posgrad Biotecnol, Feira de Santana, BA, Brazil
dc.description.affiliationUniversidade Federal da Bahia (UFBA), Fac Biol, Inst Biol, Salvador, BA, Brazil
dc.description.affiliationUNESP, Inst Biociencias, Inst Nacl Ciência & Tecnol Fisiol Comparada, Rio Claro, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-05508 São Paulo, Brazil
dc.description.affiliationUniv Fed Rio de Janeiro, Lab Eletrofisiol Cardiaca Prof Antonio Paes de Ca, Inst Biofis Carlos Chagas Filho, BR-21941 Rio de Janeiro, Brazil
dc.description.affiliationUnespUNESP, Inst Biociencias, Inst Nacl Ciência & Tecnol Fisiol Comparada, Rio Claro, SP, Brazil
dc.description.sponsorshipBrazilian Ministry of Science and Technology
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFinanciadora de Estudos e Projetos (FINEP)
dc.description.sponsorshipFundação Oswaldo Cruz (Fiocruz)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado da Bahia (FAPESB)
dc.format.extent128-136
dc.identifierhttp://dx.doi.org/10.1016/j.rppneu.2011.12.007
dc.identifier.citationRevista Portuguesa de Pneumologia. Barcelona: Elsevier Doyma Sl, v. 18, n. 3, p. 128-136, 2012.
dc.identifier.doi10.1016/j.rppneu.2011.12.007
dc.identifier.issn0873-2159
dc.identifier.urihttp://hdl.handle.net/11449/41407
dc.identifier.wosWOS:000303449100005
dc.language.isoeng
dc.publisherElsevier Doyma Sl
dc.relation.ispartofRevista Portuguesa de Pneumologia
dc.relation.ispartofjcr1.731
dc.relation.ispartofsjr0,342
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectPulmonary emphysemaen
dc.subjectErgometric testen
dc.subjectPorcine elastaseen
dc.subjectC57Bl/6en
dc.titleAlterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbancesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier Doyma Sl
unesp.author.orcid0000-0001-6099-6178[3]
unesp.author.orcid0000-0001-7549-2992[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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