Structural and morphological properties of in-situ biosynthesis of biocompatible bacterial cellulose/Laponite nanocomposites

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dc.contributor.authorDomeneguetti, Rafael R. [UNESP]
dc.contributor.authorSakai, Vanessa Y.
dc.contributor.authorPerotti, Gustavo F.
dc.contributor.authorSilva, Isabel C. [UNESP]
dc.contributor.authorTercjak, Agnieszka
dc.contributor.authorBarud, Hernane S.
dc.contributor.authorPavan, Fernando [UNESP]
dc.contributor.authorConstantino, Vera R.L.
dc.contributor.authorRibeiro, Sidney J. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal do Amazonas (UFAM)
dc.contributor.institutionUniversity of the Basque Country (UPV/EHU)
dc.contributor.institutionLaboratório de BioPolímeros e Biomateriais (BioPolMat/Uniara)
dc.date.accessioned2023-07-29T13:42:56Z
dc.date.available2023-07-29T13:42:56Z
dc.date.issued2023-03-15
dc.description.abstractBacterial cellulose (BC) membranes were produced in culture media containing Komagataeibacter xylinus microorganism and four different concentrations of Laponite (Lap) XLG up to 1.0% (m/v) to evaluate the availability of in-situ method for production of BC/Lap nanocomposites. The effect of Lap on the structure and some properties of the biosynthesised polymer was evaluated by thermogravimetric analysis coupled to mass spectrometry (TGA-MS), Fourier Transform infrared (FT-IR) and Raman (FT-Raman) vibrational spectroscopies, X-ray diffraction (XRD), scanning electron microscopy (SEM) with energy dispersive X-ray spectroscopy (EDS) and atomic force microscopy (AFM). TGA-MS and vibrational spectroscopy indicated that Lap particles were not incorporated into the BC membranes using 0.10 or 0.25% of the inorganic phase whereas all obtained results showed that its presence had a strong influence on the BC properties. This result can be an effect of the interaction of hydrophilic Lap particles with elementary fibrils of cellulose, expelled from the bacteria, fostering the disruption of chains organization. However, the 3D hierarchical organization of BC at the microscale was not collapsed according to SEM and AFM images. As the amount of Lap increased in the culture medium and in the produced membrane, the ribbons became less defined, suggesting the predominance of thinner ribbons. The transparency of the membranes was enhanced when the nanoclay loading was raised. The presence of high clay loadings in the BC/Lap nanocomposite did not negatively impact the cell viability against keratinocytes in comparison to pristine BC membranes, opening new opportunities to explore such nanocomposites for biomedical, pharmaceutical and cosmetics uses.en
dc.description.affiliationDepartamento de Química Inorgânica Instituto de Química Universidade Estadual Paulista (UNESP), Rua Prof. Francisco Degni, 55 Bairro Quitandinha, SP
dc.description.affiliationDepartamento de Química Fundamental Instituto de Química Universidade de São Paulo (USP), Av. Prof. Lineu Prestes, 748, Cidade Universitária, SP
dc.description.affiliationInstituto de Ciências Exatas e Tecnologia Universidade Federal do Amazonas (UFAM), Rua Nossa Senhora do Rosário, 3863, Bairro Tiradentes, AM
dc.description.affiliationDepartamento de Ciências Biológicas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista (UNESP), Rodovia Araraquara Jaú, Km 01 - s/n, Bairro Campos Ville, SP
dc.description.affiliationGroup ‘Materials + Technologies´ (GMT) Department of Chemical and Environmental Engineering Faculty of Engineering Gipuzkoa University of the Basque Country (UPV/EHU), Plaza Europa 1
dc.description.affiliationLaboratório de BioPolímeros e Biomateriais (BioPolMat/Uniara), Rua Carlos Gomes, 1338, Bairro Centro, SP
dc.description.affiliationUnespDepartamento de Química Inorgânica Instituto de Química Universidade Estadual Paulista (UNESP), Rua Prof. Francisco Degni, 55 Bairro Quitandinha, SP
dc.description.affiliationUnespDepartamento de Ciências Biológicas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista (UNESP), Rodovia Araraquara Jaú, Km 01 - s/n, Bairro Campos Ville, SP
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2013/07793-6
dc.description.sponsorshipIdFAPESP: 2014/50869-6
dc.description.sponsorshipIdFAPESP: 2018/25512-8
dc.description.sponsorshipIdFAPESP: 314034/2021-8
dc.description.sponsorshipIdCNPq: 407822/2018-6
dc.description.sponsorshipIdCNPq: INCT-INFO
dc.identifierhttp://dx.doi.org/10.1016/j.clay.2023.106851
dc.identifier.citationApplied Clay Science, v. 234.
dc.identifier.doi10.1016/j.clay.2023.106851
dc.identifier.issn0169-1317
dc.identifier.scopus2-s2.0-85148541547
dc.identifier.urihttp://hdl.handle.net/11449/248398
dc.language.isoeng
dc.relation.ispartofApplied Clay Science
dc.sourceScopus
dc.subjectBiopolymers
dc.subjectBiosynthesised nanocomposite
dc.subjectCellulose-synthesizing bacteria
dc.subjectHectorite
dc.subjectLayered magnesium silicate
dc.subjectPolymer-clay nanocomposites
dc.titleStructural and morphological properties of in-situ biosynthesis of biocompatible bacterial cellulose/Laponite nanocompositesen
dc.typeArtigo

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Artigos - Química Inorgânica - IQ
Artigos - Ciências Biológicas - FCFAR