Reagentless biosensor for isocitrate using one step modified Pt-Ir microelectrode

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dc.contributor.authorPereira, A. C.
dc.contributor.authorFertonani, F. L.
dc.contributor.authorNeto, G. D.
dc.contributor.authorKubota, L. T.
dc.contributor.authorYamanaka, H.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Sao Francisco
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2014-05-20T15:28:41Z
dc.date.available2014-05-20T15:28:41Z
dc.date.issued2001-01-05
dc.description.abstractThe Pt-Ir microelectrode modified through one step, electropolymerization is proposed for the isocitrate amperometric biosensor construction. The enzyme (isocitrate dehydrogenase-ICDH), coenzyme (NADP(+)) and mediator (Meldola's Blue) were immobilized onto the microelectrode surface in one step from a PIPES buffer solution containing pyrrole. The optimized experimental conditions were 25 cycles of cyclic voltammetric in a solution containing 3.58 10(-5) mol l(-1) of mediator, 3.51 10(-4) mol l(-1) of coenzyme and 2.68 U ml(-1) of enzyme. In contrast to the biosensor for isocitrate reported in literature, just one enzyme was immobilized and no coenzyme addition in the solution of analysis was necessary. Catalytic currents were proportional to the isocitrate concentration between 7.7 10(-6) and 1.04 10(-4) mol l(-1), showing good repeatability. The detection limit of the proposed biosensor was 3.50 10(-6) mol l(-1), the response time was lower than 20 s, the lifetime was about 30 determinations and no significant interference of sugars and citric acid was verified. Orange juice samples were analysed by both methodology biosensor and spectrophotometric commercial kit, and the obtained results presented a good correlation. The data demonstrated that the developed biosensor is suitable for isocitrate determination in orange juice without matrix interferences. (C) 2001 Elsevier B.V. B.V. All rights reserved.en
dc.description.affiliationUNESP, Inst Quim, BR-14801970 Araraquara, SP, Brazil
dc.description.affiliationUniv Sao Francisco, Ctr Ciências Biol & Saude, BR-12900000 Braganca Paulista, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Inst Quim, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Quim, BR-14801970 Araraquara, SP, Brazil
dc.format.extent801-806
dc.identifierhttp://dx.doi.org/10.1016/S0039-9140(00)00566-X
dc.identifier.citationTalanta. Amsterdam: Elsevier B.V., v. 53, n. 4, p. 801-806, 2001.
dc.identifier.doi10.1016/S0039-9140(00)00566-X
dc.identifier.issn0039-9140
dc.identifier.urihttp://hdl.handle.net/11449/38444
dc.identifier.wosWOS:000166393200012
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofTalanta
dc.relation.ispartofjcr4.244
dc.relation.ispartofsjr1,186
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectisocitratept
dc.subjectbiosensorpt
dc.subjectPt-Ir microelectrodept
dc.titleReagentless biosensor for isocitrate using one step modified Pt-Ir microelectrodeen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.

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