microRNA156-targeted SPL/ SBP box transcription factors regulate tomato ovary and fruit development

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dc.contributor.authorFerreira e Silva, Geraldo Felipe [UNESP]
dc.contributor.authorSilva, Eder Marques [UNESP]
dc.contributor.authorAzevedo, Mariana da Silva
dc.contributor.authorCorazon Guivin, Mike Anderson [UNESP]
dc.contributor.authorRamiro, Daniel Alves
dc.contributor.authorFigueiredo, Cassia Regina
dc.contributor.authorCarrer, Helaine
dc.contributor.authorPereira Peres, Lazaro Eustaquio
dc.contributor.authorSilveira Nogueira, Fabio Tebaldi [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-12-03T13:08:57Z
dc.date.available2014-12-03T13:08:57Z
dc.date.issued2014-05-01
dc.description.abstractFruit ripening in tomato (Solanum lycopersicum L.) is well understood at the molecular level. However, information regarding genetic pathways associated with tomato ovary and early fruit development is still lacking. Here, we investigate the possible role(s) of the microRNA156/SQUAMOSA promoter-binding protein-like (SPL or SBP box) module (miR156 node) in tomato ovary development. miR156-targeted S.lycopersicum SBP genes were dynamically expressed in developing flowers and ovaries, and miR156 was mainly expressed in meristematic tissues of the ovary, including placenta and ovules. Transgenic tomato cv. Micro-Tom plants over-expressing the AtMIR156b precursor exhibited abnormal flower and fruit morphology, with fruits characterized by growth of extra carpels and ectopic structures. Scanning electron microscopy and histological analyses showed the presence of meristem-like structures inside the ovaries, which are probably responsible for the ectopic organs. Interestingly, expression of genes associated with meristem maintenance and formation of new organs, such as LeT6/TKN2 (a KNOX-like classI gene) and GOBLET (a NAM/CUC-like gene), was induced in developing ovaries of transgenic plants as well as in the ovaries of the natural mutant Mouse ear (Me), which also displays fruits with extra carpels. Conversely, expression of the MADS box genes MACROCALYX (MC) and FUL1/TDR4, and the LEAFY ortholog FALSIFLORA, was repressed in the developing ovaries of miR156 over-expressors, suggesting similarities with Arabidopsis at this point of the miR156/SPL pathway but with distinct functional consequences in reproductive development. Altogether, these observations suggest that the miR156 node is involved in maintenance of the meristematic state of ovary tissues, thereby controlling initial steps of fleshy fruit development and determinacy.en
dc.description.affiliationState Univ Sao Paulo, Inst Biociencias, Lab Mol Genet Plant Dev, BR-18618970 Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Ctr Biotecnol Agr, Escola Super Agr Luiz de Queiroz, BR-13418900 Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Lab Hormonal Control Plant Dev, Escola Super Agr Luiz de Queiroz, Dept Biol Sci, BR-13418900 Sao Paulo, Brazil
dc.description.affiliationUnespState Univ Sao Paulo, Inst Biociencias, Lab Mol Genet Plant Dev, BR-18618970 Sao Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipBrazilian National Council for Scientific and Technological Development
dc.description.sponsorshipIdFAPESP: 09/12710-7
dc.description.sponsorshipIdFAPESP: 12/51146-2
dc.description.sponsorshipIdBrazilian National Council for Scientific and Technological Development477630/2011-1
dc.format.extent604-618
dc.identifierhttp://dx.doi.org/10.1111/tpj.12493
dc.identifier.citationPlant Journal. Hoboken: Wiley-blackwell, v. 78, n. 4, p. 604-618, 2014.
dc.identifier.doi10.1111/tpj.12493
dc.identifier.issn0960-7412
dc.identifier.urihttp://hdl.handle.net/11449/111769
dc.identifier.wosWOS:000335537600006
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofPlant Journal
dc.relation.ispartofjcr5.775
dc.relation.ispartofsjr3,582
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectmicroRNA156en
dc.subjectSPLen
dc.subjectSBPsen
dc.subjectfruit patterningen
dc.subjectgynoeciumen
dc.subjectovaryen
dc.subjectSolanum lycopersicumen
dc.titlemicroRNA156-targeted SPL/ SBP box transcription factors regulate tomato ovary and fruit developmenten
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.author.orcid0000-0002-1094-6604[3]
unesp.author.orcid0000-0002-9329-1841[7]
unesp.author.orcid0000-0001-6613-4069[9]
unesp.author.orcid0000-0002-8761-5934[8]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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