Characterization of esterase patterns in hepatopancreas of three species of Macrobrachium (Palaemonidae)

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dc.contributor.authorVidotto Barboza Lima, Alexandre [UNESP]
dc.contributor.authorGuerra, Ana Letícia [UNESP]
dc.contributor.authorAlves de Almeida, Eduardo [UNESP]
dc.contributor.authorGazzi Taddei, Fabiano
dc.contributor.authorCastiglioni, Lilian
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity Center of Rio Preto (UNIRP)
dc.date.accessioned2014-05-27T11:28:44Z
dc.date.available2014-05-27T11:28:44Z
dc.date.issued2013-04-01
dc.description.abstractThe genus Macrobrachium (Bate, 1868) belongs to the Palaemonidae family. These species are commonly found in lakes, floodplains and rivers in tropical and subtropical regions of South America. The Macrobrachium genus encompasses nearly 210 species of ecological and economic importance. In this study, three species of Macrobrachium (M acrobrachium jelskii, M acrobrachium amazonicum and M acrobrachium brasiliense) were studied in order to characterize the esterase patterns in the hepatopancreas, which were still unknown. Esterases are enzymes which catalyze the hydrolysis of esters. In the hepatopancreas, these enzymes play important roles in several metabolic processes involved in some functions of this organ, such as detoxification and digestion. Twelve esterase bands (EST1 to EST12) were detected in these species, and a comparison among them showed no qualitative differences in interspecific bands, or between males and females. Inhibitors were used to classify the esterase bands. The results indicated seven acetylesterases, two carboxylesterases, one arylesterase, and one cholinesterase. The EST11 band was not detected in these procedures because of its lower frequency. Statistical analyses showed no variability among the species, in either interspecific or intraspecific assays. These results support the hypothesis of a high evolutionary conservation of esterases in the hepatopancreas of these crustaceans. The data enabled us to assess the genetic structure of these species through the use of esterasic enzymes. It also contributes to our knowledge about the biology of these poorly studied species. Knowledge on the genetic structure of populations and species are essential when defining priorities for their management and conservation. © 2012 Elsevier Ltd.en
dc.description.affiliationDepartment of Biology, IBILCE University of São Paulo State (UNESP), Rua Cristóvão Colombo, 2265, 15054-000 São José do Rio Preto, SP
dc.description.affiliationDepartment of Biology University Center of Rio Preto (UNIRP), Rua Yvette Gabriel Atique, 45, 15025-400 São José do Rio Preto, SP
dc.description.affiliationDepartment of Chemistry and Environmental Sciences, IBILCE University of São Paulo State (UNESP), Rua Cristóvão Colombo, 2265, 15054-000 São José do Rio Preto, SP
dc.description.affiliationUnespDepartment of Biology, IBILCE University of São Paulo State (UNESP), Rua Cristóvão Colombo, 2265, 15054-000 São José do Rio Preto, SP
dc.description.affiliationUnespDepartment of Chemistry and Environmental Sciences, IBILCE University of São Paulo State (UNESP), Rua Cristóvão Colombo, 2265, 15054-000 São José do Rio Preto, SP
dc.format.extent132-138
dc.identifierhttp://dx.doi.org/10.1016/j.bse.2012.10.006
dc.identifier.citationBiochemical Systematics and Ecology, v. 47, p. 132-138.
dc.identifier.doi10.1016/j.bse.2012.10.006
dc.identifier.issn0305-1978
dc.identifier.scopus2-s2.0-84871574101
dc.identifier.urihttp://hdl.handle.net/11449/74914
dc.identifier.wosWOS:000317166400021
dc.language.isoeng
dc.relation.ispartofBiochemical Systematics and Ecology
dc.relation.ispartofjcr0.847
dc.relation.ispartofsjr0,373
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectCrustacean
dc.subjectEsterase
dc.subjectHepatopancreas
dc.subjectMacrobrachium
dc.subjectPolymorphism
dc.subjectPrawn
dc.subjectcatalysis
dc.subjectchemical ecology
dc.subjectcrustacean
dc.subjectdigestion
dc.subjectenzyme activity
dc.subjectester
dc.subjectfloodplain
dc.subjectfreshwater ecosystem
dc.subjectgenetic structure
dc.subjecthydrolysis
dc.subjectinhibitor
dc.subjectmetabolism
dc.subjectsubtropical region
dc.subjecttropical region
dc.subjectSouth America
dc.subjectCrustacea
dc.subjectPalaemonidae
dc.titleCharacterization of esterase patterns in hepatopancreas of three species of Macrobrachium (Palaemonidae)en
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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