Ozonioterapia sistêmica como terapia auxiliar na regeneração óssea guiada
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Data
2023-02-23
Autores
Baggio, Ana Maira Pereira [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
Processos regenerativos são procedimentos frequentes na odontologia atual. Entender e viabilizar esses processos associados a terapias auxiliares que melhoram ou aceleram essa prática podem ser relevantes diante dos desafios nas reconstruções. O objetivo do estudo visou avaliar o possível benefício da terapia com ozônio, quando aplicada juntamente com membranas biodegradáveis em defeitos críticos em calotas de ratos jovens na regeneração óssea guiada. Foram utilizados 96 ratos Wistar machos divididos em 4 grupos e um defeito crítico na calota de todos os animais foi realizado com 8mm de diâmetro. O Grupo BIO, teve o defeito crítico preenchido por uma membrana de colágeno porcino (BioGide®); o Grupo BIO+OZ, que somado a membrana os animais receberam a ozonioterapia sistêmica via intraperitoneal; o Grupo COA+OZ que teve o defeito preenchido pelo coágulo sanguíneo e os animais também receberam a ozonioterapia sistêmica e Grupo COA que teve o defeito crítico preenchido apenas pelo coágulo sanguíneo. Os tempos experimentais foram de 7, 15, 30 e 60 dias. As peças foram analisadas quanto à histologia, perfil inflamatório, parâmetros volumétricos pela microCT, potencial osteogênico, análise quantitativa de neoformação óssea na região do defeito e análise estatística. Os dados obtidos revelaram que os grupos tratados com a ozonioterapia atingiram resultados superiores em todos os parâmetros quando comparados aos animais que receberam apenas a membrana de colágeno porcino ou tiveram o defeito crítico preenchido apenas pelo coágulo sanguíneo. Portanto, foi possível concluir que a ozonioterapia tem influência positiva nos processos regenerativos, induz a proliferação celular e a resposta cicatricial.
Regenerative processes are frequent procedures in current dentistry. Understanding and enabling these processes associated with auxiliary therapies that improve or accelerate this practice can be relevant in the face of challenges in reconstructions. The objective of the study was to evaluate the possible benefit of ozone therapy, when applied together with biodegradable membranes in critical defects in calfs of young rats in guided bone regeneration. 96 male Wistar rats divided into 4 groups were used and a critical defect in the calvaria of all animals was performed with 8mm in diameter. The BIO Group had the critical defect filled by a porcine collagen membrane (BioGide®); the BIO+OZ Group, which added to the membrane, the animals received systemic ozone therapy intraperitoneally; the COA+OZ Group that had the defect filled by the blood clot and the animals also received systemic ozone therapy and the COA Group that had the critical defect filled only by the blood clot. The experimental times were 7, 15, 30 and 60 days. The pieces were analyzed for histology, inflammatory profile, volumetric parameters by microCT, osteogenic potential, quantitative analysis of bone neoformation in the region of the defect and statistical analysis. The data obtained revealed that the groups treated with ozone therapy achieved superior results in all parameters when compared to the animals that received only the porcine collagen membrane or had the critical defect filled only by the blood clot. Therefore, it was possible to conclude that ozone therapy has a positive influence on regenerative processes, induces cell proliferation and healing response.
Regenerative processes are frequent procedures in current dentistry. Understanding and enabling these processes associated with auxiliary therapies that improve or accelerate this practice can be relevant in the face of challenges in reconstructions. The objective of the study was to evaluate the possible benefit of ozone therapy, when applied together with biodegradable membranes in critical defects in calfs of young rats in guided bone regeneration. 96 male Wistar rats divided into 4 groups were used and a critical defect in the calvaria of all animals was performed with 8mm in diameter. The BIO Group had the critical defect filled by a porcine collagen membrane (BioGide®); the BIO+OZ Group, which added to the membrane, the animals received systemic ozone therapy intraperitoneally; the COA+OZ Group that had the defect filled by the blood clot and the animals also received systemic ozone therapy and the COA Group that had the critical defect filled only by the blood clot. The experimental times were 7, 15, 30 and 60 days. The pieces were analyzed for histology, inflammatory profile, volumetric parameters by microCT, osteogenic potential, quantitative analysis of bone neoformation in the region of the defect and statistical analysis. The data obtained revealed that the groups treated with ozone therapy achieved superior results in all parameters when compared to the animals that received only the porcine collagen membrane or had the critical defect filled only by the blood clot. Therefore, it was possible to conclude that ozone therapy has a positive influence on regenerative processes, induces cell proliferation and healing response.
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Palavras-chave
Regeneração óssea, Materiais biomédicos, Ozônio, Bone regeneration