Mutagenicity and genotoxicity of isatin in mammalian cells in vivo

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dc.contributor.authorCandido-Bacani, Priscila de Matos
dc.contributor.authordos Reis, Mariana Bisarro
dc.contributor.authorSerpeloni, Juliana Mara
dc.contributor.authorCalvo, Tamara Regina [UNESP]
dc.contributor.authorVilegas, Wagner [UNESP]
dc.contributor.authorVaranda, Eliana Aparecida [UNESP]
dc.contributor.authorde Syllos Colus, Ilce Mara
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:24:20Z
dc.date.available2014-05-20T13:24:20Z
dc.date.issued2011-02-03
dc.description.abstractIsatin (1H-indole-2,3-dione) is a synthetically versatile substrate used for the synthesis of heterocyclic compounds and as a raw material for drug synthesis. Isatin and its derivatives demonstrate anticonvulsant, antibacterial, antifungal, antiviral, and anticancer properties. We evaluated the genotoxic and mutagenic effects of acute (24h) and repeated (14d) exposure to isatin in vivo. using the cornet assay and the micronucleus test. Three doses (50, 100, and 150 mg/kg b.w.) were administered to mice via gayage. Doses were selected according to the LD(50) of isatin, estimated in a preliminary test to be 1 g/kg b.w. To evaluate the results, parametric (ANOVA/Tukey) and non-parametric (Kruskal-Wallis/Dunn's post hoc test) tests were used, according to the nature of the data distribution. At all doses (50, 100 and 150 mg/kg b.w.), after acute treatment with isatin, alterations in DNA migration (comet assay) were not observed and mutagenic effects were not seen (micronucleus test on peripheral blood cells). After repeated doses, only the highest dose of isatin (150 mg/kg b.w.) induced alterations in the DNA that gave rise to micronuclei in the bone marrow and peripheral blood cells of the mice. Our results show that the mutagenic and genotoxic effects of isatin depend on dose and on period of exposure. (C) 2010 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Estadual de Londrina (UEL), Dept Gen Biol, Ctr Biol Sci, Uel, PR, Brazil
dc.description.affiliationSão Paulo State Univ, Chem Inst Araraquara, UNESP, São Carlos, SP, Brazil
dc.description.affiliationSão Paulo State Univ, Dept Biol Sci, UNESP, Fac Pharmaceut Sci Araraquara, São Carlos, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Chem Inst Araraquara, UNESP, São Carlos, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Dept Biol Sci, UNESP, Fac Pharmaceut Sci Araraquara, São Carlos, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCandido-Bacani
dc.description.sponsorshipM.B. Reis
dc.format.extent47-51
dc.identifierhttp://dx.doi.org/10.1016/j.mrgentox.2010.11.006
dc.identifier.citationMutation Research-genetic Toxicology and Environmental Mutagenesis. Amsterdam: Elsevier B.V., v. 719, n. 1-2, p. 47-51, 2011.
dc.identifier.doi10.1016/j.mrgentox.2010.11.006
dc.identifier.fileWOS000287055900008.pdf
dc.identifier.issn1383-5718
dc.identifier.lattes7501930236496670
dc.identifier.orcid0000-0003-3032-2556
dc.identifier.urihttp://hdl.handle.net/11449/7509
dc.identifier.wosWOS:000287055900008
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofMutation Research: Genetic Toxicology and Environmental Mutagenesis
dc.relation.ispartofjcr1.996
dc.relation.ispartofsjr0,747
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectIsatinen
dc.subjectMiceen
dc.subjectMicronucleus testen
dc.subjectComet assayen
dc.titleMutagenicity and genotoxicity of isatin in mammalian cells in vivoen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes7501930236496670
unesp.author.orcid0000-0003-3032-2556[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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