Análise in vitro de técnicas e materiais utilizados clinicamente em dentística restauradora
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Data
2023-02-24
Autores
Manzoli, Tatiane Miranda
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Universidade Estadual Paulista (Unesp)
Resumo
Objetivo: Avaliar o efeito de diferentes tratamentos realizados clinicamente, a fim de apresentar contribuição de estudos in vitro com novos produtos, auxiliando o profissional na clínica odontológica. Material e métodos: No primeiro estudo, oitenta raízes de incisivos bovinos foram tratadas endodonticamente, e após o preparo para pino foi tratado de acordo com o protocolo de irrigação (DW, SHS, SHT e SHG). Em seguida, quarenta raízes (n = 10) foram utilizadas para avaliar a persistência, e as outras quarenta raízes utilizadas para o teste de resistência de união, seguido da análise do modo de falha. A formação de tags foi avaliada por imagens de microscopia confocal a laser. No segundo estudo, foram utilizadas 60 raízes de incisivos bovinos, tratados endodonticamente e então distribuídos em 6 grupo (n=10): G1-(SSAA), G2-(HCAA), G3-(HUAA), G4-(SSUU), G5-(HCUU) e G6- (HUUU). Após os tratamentos intrarradiculares, os espécimes foram preparados e submetidos aos testes de resistência de união, avaliação do padrão de falha adesiva e formação de tags. No terceiro estudo, sessenta coroas bovinas foram utilizadas. Após a coloração com chá preto, os espécimes foram randomizados em quatro grupos (n=10) de acordo com o protocolo de clareamento: HP35R: 3x15 min 35% HP; HP35: 1x 45 min 35% HP; HP35VR: 3x 8min 35% HP + LED violeta; HP35V: 1x 24 min + LED violeta. A mudança de cor foi avaliada antes, 24h após cada sessão, 7 dias e 15 dias após a última sessão. Foram realizados teste de análise de cor, variação de pH (inicial e final) e a temperatura do gel clareador. Resultados: No estudo 1, não foi observada diferença na persistência de resíduos independente do terço (p>0,05). Em relação à resistência de união, o grupo DW apresentou os maiores valores para todos os terços. No estudo 2, para o teste de resistência de união, o grupo que obteve os maiores valores foi o SSUU, enquanto o SSAA obteve valores menores no terço cervical e médio, enquanto o grupo HCAA obteve o menor valor no terço apical. Na penetrabilidade dentinária, o grupo HUAA apresentou maior extensão de tags. No estudo 3, HP35VR e HP35V tiveram a mudança de cor mais perceptível (p<0,05). Os valores finais de pH foram menores que os iniciais, mas sem diferença entre os grupos (p>0,05). Os grupos HP35VR e HP35V apresentaram aumento de temperatura em relação ao HP35R (p<0,05). Conclusão: Com base nos resultados obtidos podemos concluir que a utilização de novas formulações e técnicas tem apresentado resultados positivos, podendo ser um grande aliado nos procedimentos clínicos. No entanto, estudos in vivo precisam ser realizados para avaliação dos materiais em outras condições.
Objective: To evaluate the effect of different clinical treatments in order to present the contribution of in vitro studies with new products, helping the professional in the dental clinic. Methods: In the first study, eighty roots of bovine incisors were endodontically treated. After post-space preparation, it was irrigated according to the irrigation protocol (DW, SHS, SHT and SHG). Then, forty roots (n = 10) were used to evaluate the persistence of residues and the other roots were used for bond strength followed by failure mode analysis. Tag formation was evaluated by confocal laser microscopy images. In the second study, 60 roots of bovine incisors were endodontically treated and then distributed into 6 groups (n=10): G1-(SSAA), G2-(HCAA), G3-(HUAA), G4- (SSUU), G5-(HCUU) and G6- (HUUU). After the intraradicular treatments, the specimens were submitted to bond strength, failure mode, and tag formation tests. In the third study, sixty bovine crowns were used. After black tea staining, specimens were randomized into four groups (n=10) according to the bleaching protocol: HP35R: 3x15 min 35% HP; HP35: 1x 45 min 35% HP; HP35VR: 3x 8min 35% HP + Violet LED; HP35V: 1x 24 min + violet LED. Color change was evaluated before, 24h after each session, 7 days and 15 days after the last session. Color analysis tests, pH variation (initial and final) and temperature of the whitening gel were performed. Results: In study 1, no difference was observed in the persistence of residuals regardless of third (p > 0.05). Regarding bond strength, the DW group showed the highest values for all thirds. In study 2, for the bond strength test, the group that obtained the highest values was the SSUU, while the SSAA obtained the lowest values in the cervical and middle thirds, while the HCAA group obtained the lowest values in the apical thirds. In terms of dentin penetrability, the HUAA group had a greater number of tags. In study 3, HP35VR and HP35V had the most noticeable color change (p < 0.05). The final pH values were lower than the initial ones, but with no difference between the groups (p > 0.05). The HP35VR and HP35V groups showed an increase in temperature compared to the HP35R (p < 0.05). Conclusion: Based on the results it was possible to conclude that the use of new formulations and techniques has shown positive results, and can be useful in clinical procedures. However, in vivo studies must be carried out to evaluate the materials in other conditions.
Objective: To evaluate the effect of different clinical treatments in order to present the contribution of in vitro studies with new products, helping the professional in the dental clinic. Methods: In the first study, eighty roots of bovine incisors were endodontically treated. After post-space preparation, it was irrigated according to the irrigation protocol (DW, SHS, SHT and SHG). Then, forty roots (n = 10) were used to evaluate the persistence of residues and the other roots were used for bond strength followed by failure mode analysis. Tag formation was evaluated by confocal laser microscopy images. In the second study, 60 roots of bovine incisors were endodontically treated and then distributed into 6 groups (n=10): G1-(SSAA), G2-(HCAA), G3-(HUAA), G4- (SSUU), G5-(HCUU) and G6- (HUUU). After the intraradicular treatments, the specimens were submitted to bond strength, failure mode, and tag formation tests. In the third study, sixty bovine crowns were used. After black tea staining, specimens were randomized into four groups (n=10) according to the bleaching protocol: HP35R: 3x15 min 35% HP; HP35: 1x 45 min 35% HP; HP35VR: 3x 8min 35% HP + Violet LED; HP35V: 1x 24 min + violet LED. Color change was evaluated before, 24h after each session, 7 days and 15 days after the last session. Color analysis tests, pH variation (initial and final) and temperature of the whitening gel were performed. Results: In study 1, no difference was observed in the persistence of residuals regardless of third (p > 0.05). Regarding bond strength, the DW group showed the highest values for all thirds. In study 2, for the bond strength test, the group that obtained the highest values was the SSUU, while the SSAA obtained the lowest values in the cervical and middle thirds, while the HCAA group obtained the lowest values in the apical thirds. In terms of dentin penetrability, the HUAA group had a greater number of tags. In study 3, HP35VR and HP35V had the most noticeable color change (p < 0.05). The final pH values were lower than the initial ones, but with no difference between the groups (p > 0.05). The HP35VR and HP35V groups showed an increase in temperature compared to the HP35R (p < 0.05). Conclusion: Based on the results it was possible to conclude that the use of new formulations and techniques has shown positive results, and can be useful in clinical procedures. However, in vivo studies must be carried out to evaluate the materials in other conditions.
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Tensoativos, Azul de Metileno, Peróxido de hidrogênio, Surface-active agents, Methylene blue, Hydrogen peroxide