Occurrence and expression of p53 suppressor gene and c-Myc oncogene in dog eyelid tumors

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dc.contributor.authorLopes, Rodrigo Antonio [UNESP]
dc.contributor.authorCardoso, Tereza Cristina [UNESP]
dc.contributor.authorLuvizotto, Maria Cecília Rui [UNESP]
dc.contributor.authorAndrade, Alexandre Lima de [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:24:38Z
dc.date.available2014-05-27T11:24:38Z
dc.date.issued2010-03-01
dc.description.abstractPurpose: To detect the occurrence and expression of the suppressor gene p53 and of the oncogene c-Myc in eyelid tumors of dogs using the PCR, RT-PCR, PCR-ELISA and RT-PCR-ELISA techniques. These genes have not been described in dog eyelid tumors before. Methods: Nine samples of eyelid or third eyelid epithelial tumors were obtained from the archives of the Department of Veterinary Pathology. Tumor diagnosis was confirmed by evaluation of hematoxylin-eosin stained sections, and immunohistochemistry for cytokeratin AE1/AE3 and vimentin V9. A canine mammary tumor was used for positive control. Agarose gel electrophoresis, PCR-ELISA and RT-PCR-ELISA were used to detect p53 and c-Myc genes. Results: The occurrence of p53 was detected in most of the eyelid tumors and third eyelid tumors studied (88.8%, n = 8) and was expressed in 75% of the positive samples, as indicated by ELISA. The c-Myc gene was found in 77.7% (n = 7) of the samples and was expressed in eight samples. Conclusions: Eyelid and third eyelid tumors of dogs express both the p53 and the c-Myc genes as shown by PCR and RT-PCR. However, PCR ELISA and RT-PCR ELISA were more efficient in assessing occurrence and expression of these genes because they identified amplified products that were not detected by agarose gel electrophoresis. © 2010 American College of Veterinary Ophthalmologists.en
dc.description.affiliationPostgraduate Programme in Animal Science Faculty of Odontology Veterinary Science Course, Unesp, State of São Paulo University, Araçatuba-SP
dc.description.affiliationDepartment of Support Production and Animal Science Faculty of Odontology, Veterinary Science Course, Unesp, State of São Paulo University, Araçatuba-SP
dc.description.affiliationDepartment of Animal Clinics Surgery and Reproduction Faculty of Odontology, Veterinary Science Course, Unesp, State of São Paulo University, Araçatuba-SP
dc.description.affiliationUnespPostgraduate Programme in Animal Science Faculty of Odontology Veterinary Science Course, Unesp, State of São Paulo University, Araçatuba-SP
dc.description.affiliationUnespDepartment of Support Production and Animal Science Faculty of Odontology, Veterinary Science Course, Unesp, State of São Paulo University, Araçatuba-SP
dc.description.affiliationUnespDepartment of Animal Clinics Surgery and Reproduction Faculty of Odontology, Veterinary Science Course, Unesp, State of São Paulo University, Araçatuba-SP
dc.format.extent69-75
dc.identifierhttp://dx.doi.org/10.1111/j.1463-5224.2009.00753.x
dc.identifier.citationVeterinary Ophthalmology, v. 13, n. 2, p. 69-75, 2010.
dc.identifier.doi10.1111/j.1463-5224.2009.00753.x
dc.identifier.issn1463-5216
dc.identifier.issn1463-5224
dc.identifier.lattes4584674909952477
dc.identifier.lattes6404176495306171
dc.identifier.scopus2-s2.0-77951605799
dc.identifier.urihttp://hdl.handle.net/11449/71585
dc.language.isoeng
dc.relation.ispartofVeterinary Ophthalmology
dc.relation.ispartofjcr1.083
dc.relation.ispartofsjr0,692
dc.relation.ispartofsjr0,692
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectC-Myc
dc.subjectDog
dc.subjectELISA
dc.subjectEyelid tumors
dc.subjectP53
dc.subjectPCR
dc.subjectDNA
dc.subjectMyc protein
dc.subjectprotein p53
dc.subjectRNA
dc.subjectanimal
dc.subjectanimal disease
dc.subjectdog
dc.subjectdog disease
dc.subjecteyelid tumor
dc.subjectgene expression regulation
dc.subjectgenetics
dc.subjectmetabolism
dc.subjectphysiology
dc.subjectAnimals
dc.subjectDog Diseases
dc.subjectDogs
dc.subjectEyelid Neoplasms
dc.subjectGene Expression Regulation, Neoplastic
dc.subjectProto-Oncogene Proteins c-myc
dc.subjectTumor Suppressor Protein p53
dc.subjectCanis familiaris
dc.titleOccurrence and expression of p53 suppressor gene and c-Myc oncogene in dog eyelid tumorsen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
unesp.author.lattes4584674909952477
unesp.author.lattes6404176495306171[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatubapt

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