The biocompatibility and antifungal effect of Rosmarinus officinalis against Candida albicans in Galleria mellonella model
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2022-12-01
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Meccatti, Vanessa Marques [UNESP]
Figueiredo-Godoi, Lívia Mara Alves [UNESP]
Pereira, Thaís Cristine [UNESP]
de Lima, Patrícia Michelle Nagai [UNESP]
Abu Hasna, Amjad [UNESP]
Senna, Lavinia Barbosa [UNESP]
Marcucci, Maria Cristina [UNESP]
Junqueira, Juliana Campos [UNESP]
de Oliveira, Luciane Dias [UNESP]
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This study was performed to evaluate the biocompatibility and antifungal effect of Rosmarinus officinalis against Candida albicans in Galleria mellonella model. Five different concentrations of R. officinalis glycolic extract (50; 25; 12.5 e 6.25 mg/mL) were used to evaluate its biocompatibility in G. mellonella model, in which the nystatin suspension (100; 50; 25; 12.5 e 6.25%) was used as a control group. The antifungal action of R. officinalis glycolic extract was evaluated on C. albicans for 72, 48 and 12 h at two different phases: (1) using the extract as therapeutic agent; and (2) using the extract as prophylactic agent. PBS was used as a negative control group. G. mellonella survival curves were plotted using the Kaplan–Meier method and statistical analysis was performed using the log-rank test (Mantel–Cox) and the significance level was set at (α ≤ 0.05). There was no significant difference among the groups in which all were biocompatible except of a significant death rate of 26.6% with nystatin 100%. In phase 1, it was found that after 7 days, there was no statistically significant difference among the prophylactic treatment groups. In phase 2, the groups of R. officinalis 6.25 mg/mL for 72 h and R. officinalis of 12.5 mg/mL for 24 h promoted the survival rate of the larvae in comparison with the control group with a significant difference (p = 0.017) and (p = 0.032) respectively. Therefore, R. officinalis extract is biocompatible in different concentrations and can be used as a prophylactic agent against fungal infection.
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Scientific Reports, v. 12, n. 1, 2022.