Bacterial ectosymbionts in cuticular organs chemically protect a beetle during molting stages

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dc.contributor.authorJanke, Rebekka S.
dc.contributor.authorKaftan, Filip
dc.contributor.authorNiehs, Sarah P.
dc.contributor.authorScherlach, Kirstin
dc.contributor.authorRodrigues, Andre [UNESP]
dc.contributor.authorSvatoš, Aleš
dc.contributor.authorHertweck, Christian
dc.contributor.authorKaltenpoth, Martin
dc.contributor.authorFlórez, Laura V.
dc.contributor.institutionJohannes Gutenberg University
dc.contributor.institutionMax Planck Institute for Chemical Ecology
dc.contributor.institutionHKI
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionFriedrich Schiller University Jena
dc.contributor.institutionUniversity of Copenhagen
dc.date.accessioned2023-03-01T21:13:38Z
dc.date.available2023-03-01T21:13:38Z
dc.date.issued2022-01-01
dc.description.abstractIn invertebrates, the cuticle is the first and major protective barrier against predators and pathogen infections. While immune responses and behavioral defenses are also known to be important for insect protection, the potential of cuticle-associated microbial symbionts to aid in preventing pathogen entry during molting and throughout larval development remains unexplored. Here, we show that bacterial symbionts of the beetle Lagria villosa inhabit unusual dorsal invaginations of the insect cuticle, which remain open to the outer surface and persist throughout larval development. This specialized location enables the release of several symbiont cells and the associated protective compounds during molting. This facilitates ectosymbiont maintenance and extended defense during larval development against antagonistic fungi. One Burkholderia strain, which produces the antifungal compound lagriamide, dominates the community across all life stages, and removal of the community significantly impairs the survival probability of young larvae when exposed to different pathogenic fungi. We localize both the dominant bacterial strain and lagriamide on the surface of eggs, larvae, pupae, and on the inner surface of the molted cuticle (exuvia), supporting extended protection. These results highlight adaptations for effective defense of immature insects by cuticle-associated ectosymbionts, a potentially key advantage for a ground-dwelling insect when confronting pathogenic microbes. [Figure not available: see fulltext.]en
dc.description.affiliationDepartment of Evolutionary Ecology Institute of Organismic and Molecular Evolution Johannes Gutenberg University
dc.description.affiliationDepartment of Insect Symbiosis Max Planck Institute for Chemical Ecology
dc.description.affiliationResearch Group Mass Spectrometry Max Planck Institute for Chemical Ecology
dc.description.affiliationDepartment of Biomolecular Chemistry Leibniz Institute for Natural Products Research and Infection Biology HKI
dc.description.affiliationDepartment of Biochemistry and Microbiology UNESP-São Paulo State University
dc.description.affiliationInstitute of Microbiology Faculty of Biological Sciences Friedrich Schiller University Jena
dc.description.affiliationDepartment of Plant and Environmental Sciences Section for Organismal Biology University of Copenhagen
dc.description.affiliationUnespDepartment of Biochemistry and Microbiology UNESP-São Paulo State University
dc.identifierhttp://dx.doi.org/10.1038/s41396-022-01311-x
dc.identifier.citationISME Journal.
dc.identifier.doi10.1038/s41396-022-01311-x
dc.identifier.issn1751-7370
dc.identifier.issn1751-7362
dc.identifier.scopus2-s2.0-85137510754
dc.identifier.urihttp://hdl.handle.net/11449/241624
dc.language.isoeng
dc.relation.ispartofISME Journal
dc.sourceScopus
dc.titleBacterial ectosymbionts in cuticular organs chemically protect a beetle during molting stagesen
dc.typeArtigo
unesp.author.orcid0000-0001-6972-200X[1]
unesp.author.orcid0000-0002-4164-9362[5]
unesp.author.orcid0000-0003-1032-7288[6]
unesp.author.orcid0000-0002-0367-337X[7]
unesp.author.orcid0000-0001-9450-0345[8]
unesp.author.orcid0000-0002-0761-3729[9]

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