Avaliação da suplementação com ômega-3 como terapia preventiva a ocorrência de osteonecrose dos maxilares associada ao uso de zoledronato
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Data
2023-02-08
Autores
Turini, Halef Diego [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
Foi feita a seleção de 60 ratas ovariectomizadas que foram distribuídas seguindo uma tabela gerada por um programa computacional. Consistiu em apenas 01 grupo, com periodontite experimental induzida (PE) (n=60), subdividido em 04 subgrupos que receberam: Grupo Veículo (VEI) (n=15): quatro semanas após início da administração de VEI via intraperitoneal, os animais tiveram a PE induzida no primeiro molar inferior esquerdo. Quatorze dias após indução, foi iniciada a administração de VEI via gavagem gástrica (diária). A exodontia foi realizada 01 dia após início da administração de VEI via gavagem gástrica. A eutanásia ocorreu no 35º dia após a exodontia. Grupo Zoledronato (ZOL) (n=15): quatro semanas após início da terapia com ZOL via intraperitoneal, os animais tiveram a PE induzida no primeiro molar inferior esquerdo. Quatorze dias após indução, foi iniciada a administração de VEI via gavagem gástrica (diária). A exodontia foi realizada 01 dia após início da administração de VEI via gavagem gástrica. A eutanásia ocorreu no 35º dia após a exodontia. Grupo Ômega-3 (VEI/ω3) (n=15): quatro semanas após início da administração de VEI via intraperitoneal, os animais tiveram a PE induzida no primeiro molar inferior esquerdo. Quatorze dias após indução, foi iniciada a suplementação com ω3 via gavagem gástrica (diária). A exodontia foi realizada 01 dia após início da suplementação. A eutanásia ocorreu no 35º dia após a exodontia. Grupo Zoledronato/ômega-3 (ZOL/ω3) (n=15): quatro semanas após início da terapia com ZOL via intraperitoneal, os animais tiveram a PE induzida no primeiro molar inferior esquerdo. Quatorze dias após indução, foi iniciada a suplementação com ω3 via gavagem gástrica (diária). A exodontia foi realizada 01 dia após início da suplementação com ω3. A eutanásia ocorreu no 35º dia após a exodontia. Todas as aplicações de ZOL foram feitas com um intervalo de três dias, entretanto, a suplementação de ω3 ou VEI foram diárias, durante dez semanas e 36 dias respectivamente. Após 35 dias da exodontia, os animais foram submetidos a eutanásia pela administração de dose letal de tiopental (150 mg/kg). As mandíbulas coletadas foram divididas em duas partes e processadas de acordo com as análises propostas. As hemimandíbulas esquerdas foram utilizadas para processamento histológico para as análises histométrica, onde foi avaliado a porcentagem de tecido ósseo vital neoformado e a porcentagem de tecido ósseo não-vital. Análises imunoistoquímicas dos biomarcadores na região de alvéolo dentário (TRAP, TNF-α e IL-1β, entre outros) foram realizados. Os dados obtidos foram submetidos à análise estatística (p < 0,05) em programa computacional especializado (Bioestat® 5.0). A suplementação com ω3 parece ser benéfica frente a osteonecrose dos maxilares associada ao uso de ZOL, diminuindo a severidade dessa condição.
A selection of 60 ovariectomized rats was selected and distributed according to a table generated by a computer program. It consists of only 01 group, with experimentally induced periodontitis (EP) (n=60), subdivided into 04 subgroups that received: Vehicle Group (VEI) (n=15): four weeks after the start of intraperitoneal administration of VEI, the animals had PE induced in the lower left first molar. Fourteen days after induction, administration of VEI via gastric gavage (daily) was initiated. The extraction was performed 01 day after the beginning of the administration of VEI via gastric gavage. Euthanasia occurred on the 35th day after tooth extraction. Zoledronate (ZOL) group (n=15): four weeks after the start of intraperitoneal ZOL therapy, the animals had PE induced in the lower left first molar. Fourteen days after induction, administration of VEI via gastric gavage (daily) was initiated. The extraction was performed 01 day after the beginning of the administration of VEI via gastric gavage. Euthanasia occurred on the 35th day after tooth extraction. Omega-3 group (VEI/ω3) (n=15): four weeks after the start of intraperitoneal administration of VEI, the animals had PE induced in the lower left first molar. Fourteen days after induction, supplementation with ω3 via gastric gavage (daily) was initiated. The extraction was performed 01 day after the start of supplementation. Euthanasia occurred on the 35th day after tooth extraction. Zoledronate/omega-3 (ZOL/ω3) group (n=15): four weeks after the start of intraperitoneal ZOL therapy, the animals had PE induced in the lower left first molar. Fourteen days after induction, supplementation with ω3 via gastric gavage (daily) was initiated. The extraction was performed 01 day after the start of supplementation with ω3. Euthanasia occurred on the 35th day after tooth extraction. All ZOL applications were performed with an interval of three days, however, ω3 or VEI supplementation were daily, for ten weeks and 36 days respectively. After 35 days of extraction, the animals were euthanized with the administration of a lethal dose of thiopental (150 mg/kg). The collected mandibles were divided into two parts and processed according to the proposed analyses. The left hemimandibles were used for histological processing for histometric analysis, where the percentage of newly formed vital bone tissue and the percentage of non-vital bone tissue were evaluated. Immunohistochemical analyzes of biomarkers in the tooth socket region (TRAP, TNF-α and IL-1β, among others) were performed. The data obtained were submitted to statistical analysis (p < 0.05) in a specialized computer program (Bioestat® 5.0). Supplementation with ω3 appears to be beneficial in the face of osteonecrosis of the jaws associated with the use of ZOL, reducing the severity of this condition.
A selection of 60 ovariectomized rats was selected and distributed according to a table generated by a computer program. It consists of only 01 group, with experimentally induced periodontitis (EP) (n=60), subdivided into 04 subgroups that received: Vehicle Group (VEI) (n=15): four weeks after the start of intraperitoneal administration of VEI, the animals had PE induced in the lower left first molar. Fourteen days after induction, administration of VEI via gastric gavage (daily) was initiated. The extraction was performed 01 day after the beginning of the administration of VEI via gastric gavage. Euthanasia occurred on the 35th day after tooth extraction. Zoledronate (ZOL) group (n=15): four weeks after the start of intraperitoneal ZOL therapy, the animals had PE induced in the lower left first molar. Fourteen days after induction, administration of VEI via gastric gavage (daily) was initiated. The extraction was performed 01 day after the beginning of the administration of VEI via gastric gavage. Euthanasia occurred on the 35th day after tooth extraction. Omega-3 group (VEI/ω3) (n=15): four weeks after the start of intraperitoneal administration of VEI, the animals had PE induced in the lower left first molar. Fourteen days after induction, supplementation with ω3 via gastric gavage (daily) was initiated. The extraction was performed 01 day after the start of supplementation. Euthanasia occurred on the 35th day after tooth extraction. Zoledronate/omega-3 (ZOL/ω3) group (n=15): four weeks after the start of intraperitoneal ZOL therapy, the animals had PE induced in the lower left first molar. Fourteen days after induction, supplementation with ω3 via gastric gavage (daily) was initiated. The extraction was performed 01 day after the start of supplementation with ω3. Euthanasia occurred on the 35th day after tooth extraction. All ZOL applications were performed with an interval of three days, however, ω3 or VEI supplementation were daily, for ten weeks and 36 days respectively. After 35 days of extraction, the animals were euthanized with the administration of a lethal dose of thiopental (150 mg/kg). The collected mandibles were divided into two parts and processed according to the proposed analyses. The left hemimandibles were used for histological processing for histometric analysis, where the percentage of newly formed vital bone tissue and the percentage of non-vital bone tissue were evaluated. Immunohistochemical analyzes of biomarkers in the tooth socket region (TRAP, TNF-α and IL-1β, among others) were performed. The data obtained were submitted to statistical analysis (p < 0.05) in a specialized computer program (Bioestat® 5.0). Supplementation with ω3 appears to be beneficial in the face of osteonecrosis of the jaws associated with the use of ZOL, reducing the severity of this condition.
Descrição
Palavras-chave
Periodontite, Osteonecrose, Difosfonatos, Periodontitis