CCL3 and CXCL12 production in vitro by dental pulp fibroblasts from permanent and deciduous teeth stimulated by Porphyromonas gingivalis LPS

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dc.contributor.authorSipert, Carla Renata
dc.contributor.authorMorandini, Ana Carolina de Faria
dc.contributor.authorModena, Karin Cristina da Silva
dc.contributor.authorDionisio, Thiago Jose
dc.contributor.authorMachado, Maria Aparecida Andrade Moreira
dc.contributor.authorOliveira, Sandra Helena Penha de [UNESP]
dc.contributor.authorCampanelli, Ana Paula
dc.contributor.authorSantos, Carlos Ferreira
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:28:36Z
dc.date.available2014-05-27T11:28:36Z
dc.date.issued2013-03-01
dc.description.abstractObjective: The aim of this study was to compare the production of the chemokines CCL3 and CXCL12 by cultured dental pulp fibroblasts from permanent (PDPF) and deciduous (DDPF) teeth under stimulation by Porphyromonas gingivalis LPS (PgLPS). Material and Methods: Primary culture of fibroblasts from permanent (n=3) and deciduous (n=2) teeth were established using an explant technique. After the fourth passage, fibroblasts were stimulated by increasing concentrations of PgLPS (0 - 10 pg/mL) at 1, 6 and 24 h. The cells were tested for viability through MTT assay, and production of the chemokines CCL3 and CXCL12 was determined through ELISA. Comparisons among samples were performed using One-way ANOVA for MTT assay and Two-way ANOVA for ELISA results. Results: Cell viability was not affected by the antigen after 24 h of stimulation. PgLPS induced the production of CCL3 by dental pulp fibroblasts at similar levels for both permanent and deciduous pulp fibroblasts. Production of CXCL12, however, was significantly higher for PDPF than DDPF at 1 and 6 h. PgLPS, in turn, downregulated the production of CXCL12 by PDPF but not by DDPF. Conclusion: These data suggest that dental pulp fibroblasts from permanent and deciduous teeth may present a differential behavior under PgLPS stimulation.en
dc.description.affiliationDepartment of Biological Sciences Bauru School of Dentistry University of São Paulo, Bauru, SP
dc.description.affiliationDepartment Operative Dentistry Endodontics and Dental Materials Bauru School of Dentistry, University of São Paulo, Bauru, SP
dc.description.affiliationDepartment of Pediatric Dentistry, Orthodontics and Community Health Bauru School of Dentistry University of São Paulo, Bauru, SP
dc.description.affiliationDepartment of Basic Sciences Araçatuba School of Dentistry Univ. Estadual Paulista - UNESP, Araçatuba, SP
dc.description.affiliationUnespDepartment of Basic Sciences Araçatuba School of Dentistry Univ. Estadual Paulista - UNESP, Araçatuba, SP
dc.format.extent99-105
dc.identifierhttp://dx.doi.org/10.1590/1678-7757201300004
dc.identifier.citationJournal of Applied Oral Science, v. 21, n. 2, p. 99-105, 2013.
dc.identifier.doi10.1590/1678-7757201300004
dc.identifier.file2-s2.0-84878168654.pdf
dc.identifier.issn1678-7757
dc.identifier.issn1678-7765
dc.identifier.scielo1678-7757201300004
dc.identifier.scopus2-s2.0-84878168654
dc.identifier.urihttp://hdl.handle.net/11449/74738
dc.identifier.wosWOS:000319997700002
dc.language.isoeng
dc.relation.ispartofJournal of Applied Oral Science
dc.relation.ispartofjcr1.709
dc.relation.ispartofsjr0,645
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCCL3 chemokine
dc.subjectChemokines
dc.subjectCXCL12 chemokine
dc.subjectDental pulp
dc.subjectDental pulp inflammation
dc.subjectFibroblasts
dc.titleCCL3 and CXCL12 production in vitro by dental pulp fibroblasts from permanent and deciduous teeth stimulated by Porphyromonas gingivalis LPSen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/jaos/paboutj.htm
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araçatubapt

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