Functional luteal tissue formation and progesterone production following preovulatory follicle aspiration and follicular cell restoration in ewes
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Denadai, Renan 
Franchi, Fernanda Fagali
Garcia, Rodrigo
Salgado, Leticia Cristina
Silva, Nuno Emanuel Oliveira Figueiredo
Watanabe, Yeda Fumie
da Rocha, Paula Barreto
Oba, Eunice
Kastelic, John Patrick
Ferreira, João Carlos Pinheiro
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Undergraduate course
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Elsevier
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Abstract
The objective was to monitor corpus luteum (CL) development after preovulatory follicle aspiration, with or without intrafollicular autologous follicular cell restoration. Eighteen ewes were synchronized by inserting an intravaginal pessary with 60 mg medroxyprogesterone acetate on day -16, inducing luteolysis with cloprostenol (140 µg, im) on day -12, followed by pessary withdrawal and equine chorionic gonadotropin (400 IU, im) on day -2. Forty-eight hours later, ewes were assigned to Control (CO; n = 5), Laparoscopic aspiration (LA; n = 6), or Laparoscopic aspiration + follicular cell restoration (FCR; n = 7) treatments. Daily ultrasonography assessed the largest individual CL cross-sectional area (CLA) and total luteal sectional area (TLA), and blood samples were collected every 48 h to assess plasma progesterone concentrations. Luteal formation was observed in all ewes. However, the LA group exhibited a higher number of CLs compared to the CO group (1.7 ± 0.5 vs. 1.0 ± 0.0; P < 0.05). Nevertheless, neither CO nor LA differed from the FCR group (1.4 ± 0.2; P = 0.10 and P = 0.60, respectively). In addition, TLA values were similar among the CO, LA, and FCR groups (0.8 ± 0.04, 0.9 ± 0.05, and 1.0 ± 0.05 cm², respectively; P = 0.50) and plasma progesterone concentrations did not differ significantly (4.0 ± 0.31, 3.7 ± 0.30, and 2.9 ± 0.30 ng/mL, respectively; P = 0.30). In conclusion, preovulatory follicle aspiration induces luteal tissue formation, which, with or without intrafollicular autologous follicular cell restoration, results in functional CL capable of producing progesterone levels consistent with those normally observed during the estrous cycle.
