Biomechanics evaluation of Amazonian tambaqui (Colossoma macropomum) skin puncture after preservation with glycerol 98%

Tambaqui (Colossoma macropomum) skin represents a promising alternative for wound treatment due to its histological characteristics and mechanical properties. This study aimed to evaluate tambaqui skin preserved with glycerol 98% using mechanical puncture tests. A total of 85 tambaqui skins were randomly divided into two groups: Group 1 (G1, n = 40)-skins not subjected to glycerol preservation; Group 2 (G2, n = 45)-skins preserved in 98% glycerol. Group 2 samples were immersed in glycerol 98% and refrigerated at 5° C for 32 days. All skins underwent mechanical puncture testing to assess maximum puncture force, resistance, and deformity. Group 2 (G2) exhibited significantly higher puncture force and resistance values, and lower deformity values compared to G1. A strong correlation was found between maximum puncture force and resistance. Tambaqui (C. macropomum) skins showed greater maximum puncture strength and higher tissue resistance, with less deformity, after being preserved in glycerol 98% at a temperature of 5°C for 32 days.