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Publicação:
Two Digestive Trypsins Occur in Three Species of Neotropical Anophelines

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dc.contributor.authorCaroci, A. S.
dc.contributor.authorCalvo, E.
dc.contributor.authorRibolla, P. E. [UNESP]
dc.contributor.authorDe Bianchi, A. G.
dc.contributor.authorMarinotti, O.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversity of California
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:20:56Z
dc.date.available2014-05-27T11:20:56Z
dc.date.issued2003-11-01
dc.description.abstractTrypsin activity increases in the midgut of Anopheles aquasalis, Anopheles albitarsis, and Anopheles darlingi after a bloodmeal. The activity returns to basal levels at the time the blood is completely digested. Affinity chromatography, reversed-phase high performance liquid chromatography (HPLC), and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were used to sequentially purify the mosquito trypsins found in the midguts at 24 h after feeding. Amino-terminal sequencing of the purified trypsins showed the occurrence of two distinct trypsins in the midgut of each of the mosquitoes studied. The sequences obtained are similar to those of the trypsins of other hematophagous insects.en
dc.description.affiliationDepartamento de Parasitologia Inst. de Ciê. Bioméd. Universidade de São Paulo, São Paulo, SP, CEP 05508-900
dc.description.affiliationDept. of Molec. Biol. and Biochem. University of California, Irvine, CA 92697
dc.description.affiliationDepartamento de Parasitologia Instituto de Biociências Universidade Estadual Paulista, Botucatu, SP
dc.description.affiliationUnespDepartamento de Parasitologia Instituto de Biociências Universidade Estadual Paulista, Botucatu, SP
dc.format.extent991-995
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/14765682
dc.identifier.citationJournal of Medical Entomology, v. 40, n. 6, p. 991-995, 2003.
dc.identifier.issn0022-2585
dc.identifier.scopus2-s2.0-1642529555
dc.identifier.urihttp://hdl.handle.net/11449/67468
dc.language.isoeng
dc.relation.ispartofJournal of Medical Entomology
dc.relation.ispartofjcr1.968
dc.relation.ispartofsjr0,962
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAnopheles
dc.subjectMalaria vector
dc.subjectTrypsin
dc.subjecttrypsin
dc.subjectamino acid sequence
dc.subjectanimal
dc.subjectblood
dc.subjectBrazil
dc.subjectchemistry
dc.subjectclassification
dc.subjectcomparative study
dc.subjectdigestion
dc.subjectenzymology
dc.subjectfeeding behavior
dc.subjectgenetics
dc.subjecthuman
dc.subjectisolation and purification
dc.subjectkinetics
dc.subjectmetabolism
dc.subjectmolecular genetics
dc.subjectnucleotide sequence
dc.subjectparasitology
dc.subjectphysiology
dc.subjectsensitivity and specificity
dc.subjectsequence alignment
dc.subjectsequence homology
dc.subjectAmino Acid Sequence
dc.subjectAnimals
dc.subjectBlood
dc.subjectConserved Sequence
dc.subjectDigestion
dc.subjectFeeding Behavior
dc.subjectHumans
dc.subjectKinetics
dc.subjectMolecular Sequence Data
dc.subjectSensitivity and Specificity
dc.subjectSequence Alignment
dc.subjectSequence Homology, Amino Acid
dc.subjectAlbitarsis
dc.subjectAnimalia
dc.subjectAnopheles albitarsis
dc.subjectAnopheles aquasalis
dc.subjectAnopheles darlingi
dc.subjectHexapoda
dc.titleTwo Digestive Trypsins Occur in Three Species of Neotropical Anophelinesen
dc.typeArtigo
dcterms.licensehttp://www.ingentaconnect.com/about/terms
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatupt
unesp.departmentParasitologia - IBBpt

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Botucatu - IBB - Instituto de Biociências