Micropropagación de Pothomorphe umbellata (L.) Miq. vía organogénesis directa
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Undergraduate course
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Abstract
The present research was aimed at standardizing the protocol of seed disinfection, seed germination and organogenesis via callus of Pothomorphe umbellata (L.) Miq.The germinated seeds were inoculated in different concentrations of BAP (benzylamine purine) and NAA (naphthalene acetic acid) in order to stimulate the callus induction. After 60 days of culture, the calluses with some shoots were taken to an organogenesis medium (GA3 0.1 mg.L -1, BAP 0.5 mg.L-1) during 40 days, to be transferred later to a development medium. Finally, the plantules were acclimatized, presenting a good index of survival.
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Keywords
Growth regulators, Medicinal plants, Pothomorphe umbellata, Lepianthes peltata
Language
Spanish
Citation
Revista Cubana de Plantas Medicinales, v. 12, n. 4, 2007.
