Supported core-shell nanobiosensors for quantitative fluorescence imaging of extracellular pH
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Covalent “click” cycloaddition was used to functionalize silica substrates with pH-sensitive nanoparticles, thus producing uniform and highly luminescent analytical devices usable in both commercial fluorimeters and fluorescence microscopes. Quantitative and spatially-resolved extracellular pH measurements were successfully achieved on live cardiac fibroblasts with these novel ion-sensitive surfaces.
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Chemical Communications, v. 50, n. 89, p. 13746-13749, 2014.
