Performance characteristics of high performance liquid chromatography, first order derivative UV spectrophotometry and bioassay for fluconazole determination in capsules
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Coadvisor
Graduate program
Undergraduate course
Journal Title
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Publisher
Sociedade Brasileira de Química
Type
Article
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Acesso aberto
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Abstract
The bioassay, first order derivative UV spectrophotometry and chromatographic methods for assaying fluconazole capsules were compared. They have shown great advantages over the earlier published methods. Using the first order derivative, the UV spectrophotometry method does not suffer interference of excipients. Validation parameters such as linearity, precision, accuracy, limit of detection and limit of quantitation were determined. All methods were linear and reliable within acceptable limits for antibiotic pharmaceutical preparations being accurate, precise and reproducible. The application of each method as a routine analysis should be investigated considering cost, simplicity, equipment, solvents, speed, and application to large or small workloads.
Description
Keywords
fluconazole, liquid chromatography, bioassay
Language
English
Citation
Química Nova. Sociedade Brasileira de Química, v. 35, n. 3, p. 530-534, 2012.
