Crystallization and preliminary X-ray diffraction studies of an L-amino-acid oxidase from Lachesis muta venom
dc.contributor.author | Ullah, Anwar [UNESP] | |
dc.contributor.author | Masood, Rehana [UNESP] | |
dc.contributor.author | Spencer, Patrick Jack | |
dc.contributor.author | Murakami, Mario Tyago | |
dc.contributor.author | Arni, Raghuvir Krishnaswamy [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.contributor.institution | IPEN | |
dc.contributor.institution | Ctr Nacl Pesquisa Energia & Mat | |
dc.date.accessioned | 2015-03-18T15:55:03Z | |
dc.date.available | 2015-03-18T15:55:03Z | |
dc.date.issued | 2014-11-01 | |
dc.description.abstract | Snake-venom proteins form multi-component defence systems by the recruitment and rapid evolution of nonvenomous proteins and hence serve as model systems to understand the structural modifications that result in toxicity. l-Amino-acid oxidases (LAAOs) are encountered in a number of snake venoms and have been implicated in the inhibition of platelet aggregation, cytotoxicity, haemolysis, apoptosis and haemorrhage. An l-amino-acid oxidase from Lachesis muta venom has been purified and crystallized. The crystals belonged to space group P2(1), with unit-cell parameters a = 66.05, b = 79.41, c= 100.52 angstrom, beta = 96.55 degrees. The asymmetric unit contained two molecules and the structure has been determined and partially refined at 3.0 angstrom resolution. | en |
dc.description.affiliation | UNESP IBILCE, Dept Phys, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil | |
dc.description.affiliation | IPEN, Inst Pesquisas Energet & Nucl, Comissao Nacl Energia Nucl, BR-05508900 Sao Paulo, Brazil | |
dc.description.affiliation | Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Biociencias LNBio, BR-13083970 Campinas, Brazil | |
dc.description.affiliationUnesp | UNESP IBILCE, Dept Phys, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
dc.description.sponsorship | DAAD | |
dc.format.extent | 1556-1559 | |
dc.identifier | http://dx.doi.org/10.1107/S2053230X14017877 | |
dc.identifier.citation | Acta Crystallographica Section F-structural Biology Communications. Hoboken: Wiley-blackwell, v. 70, p. 1556-1559, 2014. | |
dc.identifier.doi | 10.1107/S2053230X14017877 | |
dc.identifier.issn | 1744-3091 | |
dc.identifier.lattes | 9162508978945887 | |
dc.identifier.orcid | 0000-0003-2460-1145 | |
dc.identifier.uri | http://hdl.handle.net/11449/117062 | |
dc.identifier.wos | WOS:000344790900021 | |
dc.language.iso | eng | |
dc.publisher | Wiley-Blackwell | |
dc.relation.ispartof | Acta Crystallographica Section F-structural Biology Communications | |
dc.rights.accessRights | Acesso restrito | |
dc.source | Web of Science | |
dc.title | Crystallization and preliminary X-ray diffraction studies of an L-amino-acid oxidase from Lachesis muta venom | en |
dc.type | Artigo | |
dcterms.license | http://olabout.wiley.com/WileyCDA/Section/id-406071.html | |
dcterms.rightsHolder | Wiley-Blackwell | |
dspace.entity.type | Publication | |
unesp.author.lattes | 9162508978945887[5] | |
unesp.author.orcid | 0000-0003-3793-7393[1] | |
unesp.author.orcid | 0000-0002-0405-8010[4] | |
unesp.author.orcid | 0000-0003-2460-1145[5] | |
unesp.campus | Universidade Estadual Paulista (UNESP), Instituto de Biociências, Letras e Ciências Exatas, São José do Rio Preto | pt |
unesp.department | Física - IBILCE | pt |